Recombinant Human Thrombopoietin/TPO Protein, Animal-Free

Name: Recombinant Human Thrombopoietin/TPO Protein, Animal-Free
Brand: R&D Systems
SKU: BT-TPO-AFL

Catalog #: BT-TPO-AFL Datasheet / COA / SDS

GMP Version Available: BT-TPO-GMP

GMP

Catalog #	Availability	Size / Price	Qty
BT-TPO-AFL-01M
BT-TPO-AFL-050

Save 15% on Select RUO Reagents. See Details.

Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human Thrombopoietin/Tpo proteins.

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All Thrombopoietin/Tpo Proteins and Enzymes

Product Details

FAQs

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Thrombopoietin/TPO Protein, Animal-Free Summary

Learn more about Animal-Free Recombinant Proteins

Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED₅₀ for this effect is 0.050-0.500 ng/mL.
The specific activity of Recombinant Human Thrombopoietin is >1 x 10⁷ units/mg, which is calibrated against the human Thrombopoietin reference standard (NIBSC code: 03/124).

Source

E. coli-derived human Thrombopoietin/Tpo protein
Ser22-Leu195
Produced using non-animal reagents in an animal-free laboratory.

Accession #

NP_000451.1

N-terminal Sequence
Analysis

Ala-Ser22

Predicted Molecular Mass

19 kDa

SDS-PAGE

18-19 kDa, under reducing conditions.

Product Datasheets

BT-TPO-AFL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT-TPO-AFL

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Equivalent bioactivity of GMP (BT-TPO-GMP), Animal-Free (Catalog # BT-TPO-AFL) and RUO (BT-TPO) grades of Recombinant Human Thrombopoietin/Tpo proteins as measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells (orange, green, red, respectively).

Bioactivity

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Animal-Free™ Recombinant Human Thrombopoietin/Tpo (Catalog # BT-TPO-AFL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line. The ED₅₀ for this effect is 0.050‑0.500 ng/mL.

SDS-PAGE

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2 μg/lane of Animal-Free™ Recombinant Human Thrombopoietin/Tpo Protein (Catalog # BT-TPO-AFL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18‑19 kDa, under reducing conditions.

Reconstitution Calculator

Background: Thrombopoietin/Tpo

Thrombopoietin (TPO) is a crucial regulator of hematopoietic stem cell (HSC) differentiation, maturation, and proliferation, as well as megakaryocytopoiesis and thrombopoiesis. TPO is often used in conjunction with Stem Cell Factor (SCF) and Flt-3 ligand in cell culture protocols to expand HSCs for bone marrow transplantation and cellular therapies. When combined with SCF and Flt-3 ligand, it promotes the differentiation of HSCs into megakaryocytes, leading to the production of platelets. It has shown promise in emerging cellular therapies for treating sickle cell disease, beta thalassemia, and other blood-related disorders. Moreover, TPO in combination with other cytokines is utilized in the generation of iNK cells, which have the potential to be incorporated into cellular immunotherapies.

Entrez Gene IDs

7066 (Human); 21832 (Mouse); 81811 (Rat)

Alternate Names

Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MKCSF; MK-CSF; ML; MPL ligand; MPLLG; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THCYT1; THPO; thrombopoietin nirs variant 1; Thrombopoietin; Tpo; TPOMKCSF

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.
For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement