RelA/NFkB p65 NLS Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-24541
![Western Blot: RelA/NFkB p65 NLS AntibodyBSA Free [NBP2-24541]](https://resources.rndsystems.com/images/products/RelA-NFkB-p65-NLS-Antibody-Western-Blot-NBP2-24541-img0008.jpg "Western Blot: RelA/NFkB p65 NLS AntibodyBSA Free [NBP2-24541]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Bovine, Equine, Primate
Cited:
Human, Mouse
Predicted:
Canine (100%), Chicken (100%), Chimpanzee (100%), Drosophila (100%), Monkey (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
A portion of the NFkB p65 NLS nuclear localization signal (NLS) (between amino acids 250-330) was used as the immunogen for this antibody.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RelA/NFkB p65 NLS Antibody - BSA Free
Western Blot: RelA/NFkB p65 NLS AntibodyBSA Free [NBP2-24541]
Western Blot: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody analysis of p65 NLS in Jurkat lysate using p65 NLS antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.![Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]](https://resources.rndsystems.com/images/products/RelA-NFkB-p65-NLS-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24541-img0011.jpg "Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]")
Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]
Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 NLS Antibody [NBP2-24541] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-RelA/NFkB p65 NLS Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]](https://resources.rndsystems.com/images/products/RelA-NFkB-p65-NLS-Antibody-Immunohistochemistry-Paraffin-NBP2-24541-img0010.jpg "Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]")
Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]
Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody Formalin-fixed, paraffin-embedded human liver stained with p65 NLS antibody at 10 ug/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.![Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]](https://resources.rndsystems.com/images/products/RelA-NFkB-p65-NLS-Antibody-Immunohistochemistry-Paraffin-NBP2-24541-img0009.jpg "Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]")
Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]
Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody analysis of p65 NLS in formalin-fixed, paraffin-embedded human HeLa cells (top left) and LPS-stimulated HeLa cells (bottom left, right) using p65 NLS antibody at 5 ug/ml.Applications for RelA/NFkB p65 NLS Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5 ug/ml
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
1-3 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RelA/NFkB p65 NLS
Alternate Names
MGC131774, NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65)), Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65, p65 NLS, p65 Nuclear Localization Sequence, p65-NLS, v-rel reticuloendotheliosis viral oncogene homolog A (avian), v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65
Gene Symbol
RELA
Additional RelA/NFkB p65 NLS Products
Product Documents for RelA/NFkB p65 NLS Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RelA/NFkB p65 NLS Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for RelA/NFkB p65 NLS Antibody - BSA Free
Powered by Bioz
Customer Reviews for RelA/NFkB p65 NLS Antibody - BSA Free
There are currently no reviews for this product. Be the first to review RelA/NFkB p65 NLS Antibody - BSA Free and earn rewards!
Have you used RelA/NFkB p65 NLS Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...