RGS14 Antibody (N133/21) - Chimeric - BSA Free

Novus Biologicals | Catalog # NBP3-52342

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # N133/21

Format

BSA Free
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Product Specifications

Immunogen

Fusion protein amino acids 1-544 (full-length) of rat RGS14 (Uniprot#: O08773) produced recombinantly in E. Coli

Specificity

Does not react with RGS12

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N133/21 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N133/21 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media.

Scientific Data Images for RGS14 Antibody (N133/21) - Chimeric - BSA Free

Immunohistochemistry-Paraffin: RGS14 Antibody (N133/21) - Chimeric - BSA Free [NBP3-52342]

Immunohistochemistry-Paraffin: RGS14 Antibody (N133/21) - Chimeric - BSA Free [NBP3-52342]

Representative section of formalin fixed, paraffin-embedded rat brain hippocampus region showing cells stained NBP3-52342 using goat anti-chicken IgY secondary antibodyb(green). DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the pyramidal neurons in the CA2 region of the hippocampus.
Western Blot: RGS14 Antibody (N133/21) - Chimeric - BSA Free [NBP3-52342]

Western Blot: RGS14 Antibody (N133/21) - Chimeric - BSA Free [NBP3-52342]

Western blotting of rat brain homogenate with RGS14 mouse monoclonal antibody (A) and NBP3-52342 (B). These antibodies were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. NBP3-52342 recognizes endogenous RGS14 with similar sensitivity as the RGS14 mouse monoclonal antibody in the rat brain homogenate at ~60 kDa

Applications for RGS14 Antibody (N133/21) - Chimeric - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

-Free Floating 1:500

Immunohistochemistry-Paraffin

1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

1X PBS (pH 7.4)

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RGS14

RGS14 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP bound form. Contains 1 GoLoco domain, 2 Ras binding (RBD) domains, 1 RGS domain.

Alternate Names

regulator of G-protein signaling 14, regulator of G-protein signalling 14

Gene Symbol

RGS14

Additional RGS14 Products

Product Documents for RGS14 Antibody (N133/21) - Chimeric - BSA Free

Certificate of Analysis

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Product Specific Notices for RGS14 Antibody (N133/21) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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