Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody
Novus Biologicals | Catalog # NB100-1553
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [NB100-1553]](https://resources.rndsystems.com/images/products/Ribosomal-Protein-S6-RPS6-[p-Ser235--p-Ser236]-Antibody-Western-Blot-NB100-1553-img0002.jpg "Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [NB100-1553]")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Ribosomal Protein S6 (GeneID 6194) around serine 235 and serine 236 according to the numbering given in entry NP_001001.2.
Modification
p Ser235, p Ser236
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody
Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [NB100-1553]
Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [NB100-1553] - Detection of Human Phospho RPS6 (S235/S236) by Western Blot. Samples: Whole cell lysate (35 mcg) from HeLa cells. Treatments consisted of growth in serum containing media (lane 1) or cultured for 24 hours in serum free media (lanes 2 to 5). At 24h, serum was replaced for 1 h (lanes 2 and 3) or serum starvation was continued (lanes 4 and 5) without rapamycin (lanes 3 and 5) with 50nM Rapamcycin (lanes 2 and 4). Antibodies: Affinity purified rabbit anti-phospho RPS6 (S235/S236) antibody NB100-1553 used at 0.04 mcg/ml (upper panel). The blot was stripped and re-blotted for total RPS6 using affinity purified rabbit anti-RPS6 antibody NB100-1595 at 0.04 mcg/ml. Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).Applications for Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Western Blot
1:2500-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Ribosomal Protein S6/RPS6
Additional Ribosomal Protein S6/RPS6 Products
Product Documents for Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
