Rif1 Antibody

Novus Biologicals | Catalog # NB300-973

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Rif1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CEEGIIDANKTETNT corresponding to internal region according to NP_060621.3.

Specificity

This antibody is expected to recognise an epitope corresponding to aa 2076-2089 of human RIF1 protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rif1 Antibody

Immunohistochemistry-Paraffin: Rif1 Antibody [NB300-973]

Immunohistochemistry-Paraffin: Rif1 Antibody [NB300-973]

Immunohistochemistry-Paraffin: Rif1 Antibody [NB300-973] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Rif1 Antibody [NB300-973]

Immunohistochemistry-Paraffin: Rif1 Antibody [NB300-973]

Immunohistochemistry-Paraffin: Rif1 Antibody [NB300-973] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Rif1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000
Application Notes
WB: Not yet tested - Our standard WB protocol would not expect to reliably detect proteins as large as the predicted MW of approx. 247 kDa band according to NP_060621.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rif1

Rif1 (Rap1-interacting factor homolog) is a protein whose primary function is in the DNA-damage response, RIF1 forms foci that colocalize with other DNA damage response factors, after induction of double-strand breaks. Rif1 localizes to double strand breaks in an ATM (ataxia telangiectasia mutated)- and 53BP1-dependent manner and functions in the intra-S-phase checkpoint that serves to slow down DNA synthesis when DNA damage has occurred.

Alternate Names

DKFZp434D1026, DKFZp781N1478, FLJ10599, FLJ12870, RAP1 interacting factor homolog (yeast), Rap1-interacting factor 1 homolog, telomere-associated protein RIF1

Entrez Gene IDs

55183 (Human)

Gene Symbol

RIF1

UniProt

NP_060621 (Human)

Additional Rif1 Products

Product Documents for Rif1 Antibody

Certificate of Analysis

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Product Specific Notices for Rif1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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