RIG-I Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-76732
![Western Blot: RIG-I AntibodyBSA Free [NBP1-76732]](https://resources.rndsystems.com/images/products/RIG-I-Antibody-Western-Blot-NBP1-76732-img0004.jpg "Western Blot: RIG-I AntibodyBSA Free [NBP1-76732]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against human GST-tagged RIG-1 protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RIG-I Antibody - BSA Free
Western Blot: RIG-I AntibodyBSA Free [NBP1-76732]
Western Blot: RIG-I Antibody [NBP1-76732] - Western blot analysis of RIG-I in Lane 1) A20 cell lysate and Lane 2) Rat heart tissue lysate with RIG-I antibody [NBP1-76732] at 2 ug/mL.![Immunohistochemistry: RIG-I Antibody - BSA Free [NBP1-76732]](https://resources.rndsystems.com/images/products/RIG-I-Antibody-Immunohistochemistry-NBP1-76732-img0005.jpg "Immunohistochemistry: RIG-I Antibody - BSA Free [NBP1-76732]")
Immunohistochemistry: RIG-I Antibody - BSA Free [NBP1-76732]
Immunohistochemistry: RIG-I Antibody [NBP1-76732] - Immunohistochemistry of RIG-I in mouse testis tissue with RIG-I Antibody NBP1-76732 at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] -
Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] - Immunofluorescence of RIG-I in rat testis tissue with RIG-I Antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] -
Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] - Immunofluorescence of RIG-I in mouse testis tissue with RIG-I Antibody at 20 ug/mL.
Immunohistochemistry: RIG-I Antibody - BSA Free [NBP1-76732] -
Immunohistochemistry: RIG-I Antibody - BSA Free [NBP1-76732] - Immunohistochemistry of RIG-I in rat heart tissue with RIG-I Antibody at 10 ug/mL.
Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] -
Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] - Immunofluorescence of RIG-I in human testis tissue with RIG-I Antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] -
Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] - Immunofluorescence of RIG-I in MCF7 cells with RIG-I Antibody at 20 ug/mL.
Western Blot: RIG-I Antibody - BSA Free [NBP1-76732] -
ADAR1 knockdown results in dsRNA increases, type I interferon signaling and pro-inflammatory protein expression. (A) Immunoblots for MDA5 and RIG-I following ADAR1 knockdown. Unpaired t-test; n = 6/condition. (B) Representative images and relative J2 (dsRNA antibody) immunofluorescence signal showing dsRNA accumulation following ADAR1 knockdown. (C) Representative immunofluorescence images showing co-localization of dsRNA (J2) and MDA5 after transfection with ADAR1 siRNA. (D) Summary immunoblot data for ICAM-1, TNF-alpha, pIRF3, pNF-kappa B, and (E) representative images following ADAR1 knockdown. (F) Concentrations of CXCL10 and IFN beta following ADAR1 siRNA knockdown. **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 in unpaired t-test; n = 9/condition. Error bars represent SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38035265), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for RIG-I Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Simple Western
1:50
Western Blot
0.5-2 ug/ml
Application Notes
See Simple Western Antibody Database for Simple Western validation: separated by Size, antibody dilution of 1:50
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RIG-I
Additional RIG-I Products
Product Documents for RIG-I Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RIG-I Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for RIG-I Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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