RPL8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35097

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RPL8 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-257 of human RPL8 (NP_000964.1).

Sequence:
MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQEKEN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPL8 Antibody - BSA Free

RPL8 Antibody

Immunohistochemistry: RPL8 Antibody [NBP3-35097] -

Immunohistochemistry: RPL8 Antibody [NBP3-35097] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using RPL8 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
RPL8 Antibody

Immunohistochemistry: RPL8 Antibody [NBP3-35097] -

Immunohistochemistry: RPL8 Antibody [NBP3-35097] - Immunohistochemistry analysis of paraffin-embedded Rat brain using RPL8 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
RPL8 Antibody

Immunocytochemistry/ Immunofluorescence: RPL8 Antibody [NBP3-35097] -

Immunocytochemistry/ Immunofluorescence: RPL8 Antibody [NBP3-35097] - Confocal immunofluorescence analysis of HeLa cells using RPL8 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.
RPL8 Antibody

Immunohistochemistry: RPL8 Antibody [NBP3-35097] -

Immunohistochemistry: RPL8 Antibody [NBP3-35097] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using RPL8 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
RPL8 Antibody

Immunocytochemistry/ Immunofluorescence: RPL8 Antibody [NBP3-35097] -

Immunocytochemistry/ Immunofluorescence: RPL8 Antibody [NBP3-35097] - Immunofluorescence analysis of HeLa cells using RPL8 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
RPL8 Antibody

Western Blot: RPL8 Antibody [NBP3-35097] -

Western Blot: RPL8 Antibody [NBP3-35097] - Western blot analysis of various lysates using RPL8 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Applications for RPL8 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:1000 - 1:4000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPL8

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Alternate Names

ribosomal protein L8,60S ribosomal protein L8

Gene Symbol

RPL8

Additional RPL8 Products

Product Documents for RPL8 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RPL8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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