RSK1 [p Ser380] Antibody (23GB2425)

Novus Biologicals | Catalog # NBP3-51183

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2425
View all RSK1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-RSK1(S380) (Uniprot: Q15418)

Modification

p Ser380

Specificity

This antibody detects endogenous levels of RPS6KA1 only when phosphorylated at S380

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RSK1 [p Ser380] Antibody (23GB2425)

RSK1 [p Ser380] Antibody (23GB2425)

Flow Cytometry: RSK1 [p Ser380] Antibody (23GB2425)[NBP3-51183] -

Flow cytometric analysis of RSK1expression in C2C12 cells using RSK1 antibody (1:2,000). Green, isotype control; red, Phospho-RSK1(S380).

Applications for RSK1 [p Ser380] Antibody (23GB2425)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RSK1

Rsk-1 (p90-Rsk-1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-Rsk-1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4).

Long Name

Ribosomal Protein S6 Kinase I

Alternate Names

HU-1, MAPKAPL1A, RPS6KA1

Gene Symbol

RPS6KA1

Additional RSK1 Products

Product Documents for RSK1 [p Ser380] Antibody (23GB2425)

Certificate of Analysis

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Product Specific Notices for RSK1 [p Ser380] Antibody (23GB2425)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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