SATB2 Antibody (CL0320)

Novus Biologicals | Catalog # NBP3-52448

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL0320
View all SATB2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9UPW6, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SATB2 Antibody (CL0320)

SATB2 Antibody (CL0320) Immunohistochemistry-Paraffin: PCM1 Antibody (CL0206) [NBP3-52444]

Immunohistochemistry-Paraffin: PCM1 Antibody (CL0206) [NBP3-52444]

Staining of U-138 MG cells, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
SATB2 Antibody (CL0320) Western Blot: SATB2 Antibody (CL0320) [NBP3-52448]

Western Blot: SATB2 Antibody (CL0320) [NBP3-52448]

Staining of human cerebral cortex shows strong nuclear positivity in neurons.
SATB2 Antibody (CL0320) Western Blot: SATB2 Antibody (CL0320) [NBP3-52448]

Western Blot: SATB2 Antibody (CL0320) [NBP3-52448]

Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.
SATB2 Antibody (CL0320) Western Blot: SOX11 Antibody (CL0142) [NBP3-52441]

Western Blot: SOX11 Antibody (CL0142) [NBP3-52441]

Staining of human liver shows no positivity in hepatocytes as expected.
SATB2 Antibody (CL0320) Western Blot: SOX11 Antibody (CL0143) [NBP3-52442]

Western Blot: SOX11 Antibody (CL0143) [NBP3-52442]

Staining of human rectum shows strong nuclear positivity in glandular cells.
SATB2 Antibody (CL0320) Western Blot: PCM1 Antibody (CL0206) [NBP3-52444]

Western Blot: PCM1 Antibody (CL0206) [NBP3-52444]

Staining of rat hippocampus shows moderate to strong nuclear positivity in CA1 layer neurons.
SATB2 Antibody (CL0320) Western Blot: Aquaporin-4 Antibody (CL0178) [NBP3-52443]

Western Blot: Aquaporin-4 Antibody (CL0178) [NBP3-52443]

Staining of rat hippocampus shows no positivity in neurons in the dentate gyrus as expected.
SATB2 Antibody (CL0320) Western Blot: Syntaxin 7 Antibody (CL0257) [NBP3-52445]

Western Blot: Syntaxin 7 Antibody (CL0257) [NBP3-52445]

Analysis in human rectum and liver tissues. Corresponding RNA-seq data are presented for the same tissues.
SATB2 Antibody (CL0320) Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP3-52446]

Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP3-52446]

Lane 1: Marker [kDa]
Lane 2: Human cell line HEL
SATB2 Antibody (CL0320) Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP3-52447]

Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP3-52447]

Lane 1: Marker [kDa]
Lane 2: Negative control (vector only transfected HEK293T lysate) 
Lane 3: SATB2 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells, LY414656)

Applications for SATB2 Antibody (CL0320)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SATB2

SATB2 binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and

Alternate Names

DNA-binding protein SATB2, FLJ32076, KIAA1034FLJ21474, MGC119474, MGC119477, SATB family member 2, SATB homeobox 2, Special AT-rich sequence-binding protein 2

Gene Symbol

SATB2

Additional SATB2 Products

Product Documents for SATB2 Antibody (CL0320)

Certificate of Analysis

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Product Specific Notices for SATB2 Antibody (CL0320)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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