SF3A2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-47281

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SF3A2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SSSESNRDRRERLRQLALETIDINKDPYFMKNHLGSYECKLCLTLHNNEGSYLAHTQGKKHQTNLARRAAKEAKEAPAQPAPEKVKVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SF3A2 Antibody - BSA Free

Western Blot: SF3A2 Antibody [NBP2-47281]

Western Blot: SF3A2 Antibody [NBP2-47281]

Western Blot: SF3A2 Antibody [NBP2-47281] - Analysis using Anti-SF3A2 antibody NBP2-47281 (A) shows similar pattern to independent antibody NBP2-48775 (B).
Immunocytochemistry/ Immunofluorescence: SF3A2 Antibody [NBP2-47281]

Immunocytochemistry/ Immunofluorescence: SF3A2 Antibody [NBP2-47281]

Immunocytochemistry/Immunofluorescence: SF3A2 Antibody [NBP2-47281] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281]

Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281]

Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281] - Staining of human kidney.
Western Blot: SF3A2 Antibody [NBP2-47281]

Western Blot: SF3A2 Antibody [NBP2-47281]

Western Blot: SF3A2 Antibody [NBP2-47281] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunohistochemistry: SF3A2 Antibody [NBP2-47281]

Immunohistochemistry: SF3A2 Antibody [NBP2-47281]

Immunohistochemistry: SF3A2 Antibody [NBP2-47281] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281]

Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281]

Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281] - Staining of human cerebral cortex, colon, kidney and testis using Anti-SF3A2 antibody NBP2-47281 (A) shows similar protein distribution across tissues to independent antibody NBP2-48775 (B).
Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281]

Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281]

Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281] - Staining of human testis.
Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281]

Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281]

Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281]

Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281]

Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281] - Staining of human colon.

Applications for SF3A2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SF3A2

SF3A2 encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein.

Alternate Names

pre-mRNA splicing factor SF3A, subunit 2, PRP11, PRPF11, SAP 62, SAP62Prp11, SF3a66splicing factor 3a, subunit 2, 66kD, spliceosome associated protein 62, Spliceosome-associated protein 62, splicing factor 3A subunit 2, splicing factor 3a, subunit 2, 66kDa

Gene Symbol

SF3A2

Additional SF3A2 Products

Product Documents for SF3A2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SF3A2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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