SF3B2 Antibody

Novus Biologicals | Catalog # NB100-79847

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SF3B2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 775 and 825 of human splicing factor 3b, subunit 2, 145kDa (spliceosome associated protein 145) using the numbering given in entry AAA97461.1 (GeneID 10992).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SF3B2 Antibody

Western Blot: SF3B2 Antibody [NB100-79847]

Western Blot: SF3B2 Antibody [NB100-79847]

Western Blot: SF3B2 Antibody [NB100-79847] - Detection of Human SF3b145/SAP145 on HeLa whole cell lysate using NB100-79847. SF3b145/SAP145 was also immunoprecipitated by rabbit anti-SF3b145/SAP145 antibody NB100-79848.
Immunohistochemistry: SF3B2 Antibody [NB100-79847]

Immunohistochemistry: SF3B2 Antibody [NB100-79847]

Immunohistochemistry: SF3B2 Antibody [NB100-79847] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SF3b145/SAP145 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: SF3B2 Antibody [NB100-79847]

Western Blot: SF3B2 Antibody [NB100-79847]

Western Blot: SF3B2 Antibody [NB100-79847] - Analysis of SF3B2 in HeLa whole cell lysate using anti- SF3B2 antibody. The primary antibody was used at a dilution of 1:500 with overnight incubation at 4C. Image from verified customer review.

Applications for SF3B2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 mcg/mg lysate

Western Blot

1:2000-1:100000
Application Notes
Immunohistochemistry 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 4 using NB100-79847 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SF3B2

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.

Alternate Names

Cus1, pre-mRNA splicing factor SF3b 145 kDa subunit, Pre-mRNA-splicing factor SF3b 145 kDa subunit, SAP145SAP 145, SF3b1, SF3B145, SF3b150, spliceosome associated protein 145, Spliceosome-associated protein 145, splicing factor 3B subunit 2, splicing factor 3b, subunit 2, 145kD, splicing factor 3b, subunit 2, 145kDa

Entrez Gene IDs

10992 (Human)

Gene Symbol

SF3B2

UniProt

Q13435 (Human)

Additional SF3B2 Products

Product Documents for SF3B2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SF3B2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for SF3B2 Antibody (1)

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1 Customer Rating
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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 10/16/2015
    Hela cells lysate 25 ug protein loaded on SDS gel (6%) used Tris Glycine for electrophoresis. Major bands ~145 kDa
    SF3B2 Antibody NB100-79847

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Protocols

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