SH3PX1 Antibody (24GB13195)

Novus Biologicals | Catalog # NBP3-51892

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 24GB13195
View all SH3PX1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human SH3PX1 (Uniprot: Q9Y5X1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SH3PX1 Antibody (24GB13195)

SH3PX1 Antibody (24GB13195)

Immunocytochemistry/ Immunofluorescence: SH3PX1 Antibody (24GB13195)[NBP3-51892] -

SH3PX1 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: SNX9 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SNX9 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
SH3PX1 Antibody (24GB13195)

Immunocytochemistry/ Immunofluorescence: SH3PX1 Antibody (24GB13195)[NBP3-51892] -

Immunocytochemical staining of Hela cells with SH3PX1 antibody (1:1,000). Nuclei were stained blue with DAPI; SH3PX1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
SH3PX1 Antibody (24GB13195)

Flow Cytometry: SH3PX1 Antibody (24GB13195)[NBP3-51892] -

Validation of SH3PX1 knockdown using flow cytometry. Wild-type(WT, Blue) and SH3PX1 knockdown(KD, Green) HeLa cells were stained with SH3PX1 antibody(1:2,000) and analyzed using BD flow cytometer.
SH3PX1 Antibody (24GB13195)

Flow Cytometry: SH3PX1 Antibody (24GB13195)[NBP3-51892] -

Flow cytometric analysis of SH3PX1expression in HeLa cells using SH3PX1 antibody (1:2,000). Green, isotype control; red, SNX9.

Applications for SH3PX1 Antibody (24GB13195)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SH3PX1

The SH3PX1 (SNX1) gene is part of the sorting nexin family, thus plays a role in several states of intracellular trafficking. There is only one isoform at 595 amino acids long and at a mass of around 66 kDA. The SH3PX1 gene has been linked to choanal atresia, alcoholism, asthma, rabies, neuronitis, and wiskitt-aldrich syndrome. It is involved in clathrin derived and trans-Golgi network vesicle budding as well as membrane trafficking through interactions with other genes such as ITCH, DNM2, SYNJ1, ASAP1, and ADAM15.

Alternate Names

MST155, MSTP155, Protein SDP1, SDP1, SH3 and PX domain-containing protein 1, SH3PX1WISP, SH3PXD3ASH3 and PX domain-containing protein SH3PX1, sorting nexin 9, sorting nexin-9, WiskSH3 and PX domain-containing protein 3A

Gene Symbol

SNX9

Additional SH3PX1 Products

Product Documents for SH3PX1 Antibody (24GB13195)

Certificate of Analysis

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Product Specific Notices for SH3PX1 Antibody (24GB13195)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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