SHIP Antibody (SHIP-01) - BSA Free

Western Blot Shows Human SHIP Specificity Using Knockout Cell Line.

Western blot shows lysates of iPSC-derived microglia and SHIP knockout iPSC-derived microglia (KO). Nitrocellulose membrane was probed with SHIP Antibody (SHIP-01) - BSA Free (Catalog # NB110-81538) followed by HRP-conjugated goat anti-mouse IgG Secondary Antibody. A specific band was detected for SHIP at approximately 135 kDa (as indicated) in the parental iPSC-derived microglia cell line, but is not detectable in knockout iPSC-derived microglia cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Western Blot: SHIP Antibody (SHIP-01)BSA Free [NB110-81538]

Western Blot: SHIP Antibody (SHIP-01) [NB110-81538] - Analysis (reducing conditions) of human SHIP-1 in whole cell lysate of THP-1 human acute monocytic leukemia cell line. Lane 1: immunostaining with Isotype mouse IgG1 control Lane 2,3: immunostaining with anti-human SHIP-1 (SHIP-01) Lane 4,5: immunostaining with anti-human SHIP-1 (SHIP-02).

Flow Cytometry: Mouse Monoclonal SHIP Antibody (SHIP-01) [NB110-81538] -

Flow Cytometry: Mouse Monoclonal SHIP Antibody (SHIP-01) [NB110-81538] - Separation of MOLT-4 cells (red-filled) from CaCo-2 cells (black-dashed) in flow cytometry analysis (intracellular staining) of cellular suspensions of MOLT-4 and CaCo-2 cell lines stained using anti-SHIP (SHIP-01) purified antibody (concentration in sample 1 ug/ml, GAM APC).