Sirtuin 3/SIRT3 Antibody (7O4G7)
Novus Biologicals | Catalog # NBP3-16083
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083]](https://resources.rndsystems.com/images/products/Sirtuin-3-SIRT3-Antibody-7O4G7-Immunohistochemistry-Paraffin-NBP3-16083-img0002.jpg "Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7O4G7 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-399 of human Sirtuin 3/SIRT3 (NP_036371.1). QRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Sirtuin 3/SIRT3 Antibody (7O4G7)
Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083]
Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] - Immunohistochemistry of paraffin-embedded mouse kidney using [KO Validated] Sirtuin 3/SIRT3 Rabbit mAb (NBP3-16083) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western Blot: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] -
Western Blot: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] - Western blot analysis of lysates from wild type (WT) and Sirtuin 3/SIRT3 knockout (KO) 293T cells using [KO Validated] Sirtuin 3/SIRT3 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western Blot: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] -
Western Blot: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] - Western blot analysis of various lysates using [KO Validated] Sirtuin 3/SIRT3 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunoprecipitation: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] -
Immunoprecipitation: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] - Immunoprecipitation analysis of 300ug extracts of Hela cells using 3ug Sirtuin 3/SIRT3 Rabbit mAb. Western blot was performed from the immunoprecipitate using Sirtuin 3/SIRT3 Rabbit mAb at a dilition of 1:1000.Applications for Sirtuin 3/SIRT3 Antibody (7O4G7)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Sirtuin 3/SIRT3
Long Name
Silent Mating Type Information Regulation 3 Homolog
Alternate Names
SIR2-like protein 3, SIR2L3, SIRT3
Gene Symbol
SIRT3
Additional Sirtuin 3/SIRT3 Products
Product Documents for Sirtuin 3/SIRT3 Antibody (7O4G7)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Sirtuin 3/SIRT3 Antibody (7O4G7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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