SLTM Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38464

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: STDTPNKKPTKGKGKKHEADELSGDASVEDDAFIKDCELENQEAHEQDGNDELKDSEEFGENEEENVHSKELLSAEENKRAHELIEAEGIEDIEKEDIESQEIEAQEGEDD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SLTM Antibody - BSA Free

Western Blot: SLTM Antibody [NBP2-38464]

Western Blot: SLTM Antibody [NBP2-38464]

Western Blot: SLTM Antibody [NBP2-38464] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: SLTM Antibody [NBP2-38464]

Immunocytochemistry/ Immunofluorescence: SLTM Antibody [NBP2-38464]

Immunocytochemistry/Immunofluorescence: SLTM Antibody [NBP2-38464] - Staining of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SLTM Antibody [NBP2-38464]

Immunohistochemistry-Paraffin: SLTM Antibody [NBP2-38464]

Immunohistochemistry-Paraffin: SLTM Antibody [NBP2-38464] - Staining of human rectum shows strong nuclear positivity in glandular cells.
SLTM Antibody - BSA Free

Western Blot: SLTM Antibody - BSA Free [NBP2-38464] -

Investigation of protein expression and localizationA, BWestern immunoblots for phosphorylated pyruvate dehydrogenase at pS293 (PDH; n = 6, each replicate consists of a pool of 3–4 EHTs from 2 different separate batches), AMP‐dependent protein kinase at Thr172 (AMPK; n = 6, each replicate consists of a pool of 3–4 EHTs from 2 different separate batches), galectin‐3 (n = 3; each replicate consists of a pool of 3–4 EHTs from 2 different separate batches), SAFB‐like transcription modulator (SLTM; n = 3; each replicate consists of a pool of 3–4 EHTs from 2 different separate batches), LIM and cysteine‐rich domains 1 (LMCD1; n = 3; each replicate consists of a pool of 3–4 EHTs from 2 different separate batches), calnexin (n = 6; each replicate consists of a pool of 3–4 EHTs from 2 different separate batches), and reticulocalbin‐3 (RCN‐3; n = 3; each replicate consists of a pool of 3–4 EHTs from 2 different separate batches); Coomassie staining was used as a loading control, mean +/- SEM, unpaired two‐sided Student's t‐test, *P < 0.05.CImmunofluorescence of 2D hiPSC‐CM from PLNic and PLN p.Arg14del with antibodies recognizing calnexin (green), alpha ‐actinin (red), and DAPI staining for nuclei (blue); scale bar 20 um.Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33998164), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SLTM Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLTM

When overexpressed, acts as a general inhibitor of transcription that eventually leads to apoptosis (Bysimilarity)

Alternate Names

SAFB-like, transcription modulator

Gene Symbol

SLTM

UniProt

Additional SLTM Products

Product Documents for SLTM Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SLTM Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SLTM Antibody - BSA Free

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Protocols

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FAQs

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