SMARCAD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85023

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SMARCAD1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HGEESNESAESSSNWEKQESIVLKLQKEFPNFDKQELREVLKEHEWMYTEALESLKVFAEDQDMQYVSQSEVPNGKEVSSRSQNYPKNATKTKLKQKFSMKAQNGFNKK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMARCAD1 Antibody - BSA Free

Western Blot: SMARCAD1 Antibody [NBP1-85023]

Western Blot: SMARCAD1 Antibody [NBP1-85023]

Western Blot: SMARCAD1 Antibody [NBP1-85023] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SMARCAD1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: SMARCAD1 Antibody [NBP1-85023]

Western Blot: SMARCAD1 Antibody [NBP1-85023]

Western Blot: SMARCAD1 Antibody [NBP1-85023] - Analysis in human cell line MOLT-4.
Western Blot: SMARCAD1 Antibody [NBP1-85023]

Western Blot: SMARCAD1 Antibody [NBP1-85023]

Western Blot: SMARCAD1 Antibody [NBP1-85023] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: SMARCAD1 Antibody [NBP1-85023]

Immunocytochemistry/ Immunofluorescence: SMARCAD1 Antibody [NBP1-85023]

Immunocytochemistry/Immunofluorescence: SMARCAD1 Antibody [NBP1-85023] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023]

Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023]

Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023]

Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023]

Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023]

Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023]

Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023] - Staining of human duodenum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023]

Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023]

Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023] - Staining of human liver shows no positivity in hepatocytes as expected.

Applications for SMARCAD1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMARCAD1

The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. SMARCAD1/ETL1 is a unique member of this family in that it contains two DEAD/H box motifs, and thus is also a member of the DEAD/H-box-containing helicase superfamily. The chromosomal position of SMARCAD1 has been mapped, and it resides in a region rich in breakpoints and deletions associated with several human diseases. It is suggested to play a role in genetic instability and development.

Alternate Names

ATP-dependent helicase 1, DKFZP762K2015, EC 3.6.1, EC 3.6.4.12, HEL1, hHEL1, KIAA1122ETL1DKFZp762K2015, subfamily a, containing DEAD/H box 1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A containing DEAD/H box 1, SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin

Gene Symbol

SMARCAD1

Additional SMARCAD1 Products

Product Documents for SMARCAD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SMARCAD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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