SMG1 Antibody (1A8) - Azide and BSA Free

Novus Biologicals | Catalog # H00023049-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A8

Format

Azide and BSA Free
View all SMG1 Primary Antibodies »

Product Specifications

Immunogen

SMG1 (NP_055535, 2922 a.a. ~ 3031 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Reactivity Notes

Other species not tested.

Specificity

SMG1 (1A8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SMG1 Antibody (1A8) - Azide and BSA Free

Western Blot: SMG1 Antibody (1A8) [H00023049-M02]

Western Blot: SMG1 Antibody (1A8) [H00023049-M02]

Western Blot: SMG1 Antibody (1A8) [H00023049-M02] - SMG1 monoclonal antibody (M02), clone 1A8 Analysis of SMG1 expression in Hela S3 NE.
Immunohistochemistry-Paraffin: SMG1 Antibody (1A8) [H00023049-M02]

Immunohistochemistry-Paraffin: SMG1 Antibody (1A8) [H00023049-M02]

Immunohistochemistry-Paraffin: SMG1 Antibody (1A8) [H00023049-M02] - Analysis of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Sandwich ELISA: SMG1 Antibody (1A8) [H00023049-M02] - Detection limit for recombinant GST tagged SMG1 is approximately 1ng/ml as a capture antibody.

Applications for SMG1 Antibody (1A8) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SMG1

This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternative spliced transcript variants have been described, but their full-length natures have not been determined.

Alternate Names

ATXserine/threonine-protein kinase SMG1, EC 2.7.11, EC 2.7.11.1, hSMG-1,61E3.4, KIAA0421phosphatidylinositol 3-kinase-related protein kinase, Lambda/iota protein kinase C-interacting protein, Lambda-interacting protein, LIPPI-3-kinase-related kinase SMG-1, phosphatidylinositol 3-kinase-related kinase, SMG-1, SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)

Entrez Gene IDs

23049 (Human)

Gene Symbol

SMG1

OMIM

607032 (Human)

UniProt

NP_054725.1 (Human)

Additional SMG1 Products

Product Documents for SMG1 Antibody (1A8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SMG1 Antibody (1A8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SMG1 Antibody (1A8) - Azide and BSA Free

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Protocols

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FAQs

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