SOD1/Cu-Zn SOD Antibody

Novus Biologicals | Catalog # NBP1-31204

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Cited:

Human, Bovine

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry-Frozen, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SOD1/Cu-Zn SOD Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SOD1/Cu-Zn SOD. The exact sequence is proprietary.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 30738665). Porcine reactivity reported from a verified customer review.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SOD1/Cu-Zn SOD Antibody

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Sample (50 ug of whole cell lysate) A: Rat brain 15% SDS PAGE; antibody diluted at 1:1000.
Knockout Validated: SOD1/Cu-Zn SOD Antibody [NBP1-31204]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody.
SOD1/Cu-Zn SOD Antibody

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204] -

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1/Cu-Zn SOD antibody (NBP1-31204) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SOD1/Cu-Zn SOD Antibody

Application
Recommended Usage

Immunohistochemistry-Frozen

Assay dependent

Western Blot

1:500-1:10000

Reviewed Applications

Read 1 review rated 5 using NBP1-31204 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SOD1/Cu-Zn SOD

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD1), mitochondrial manganese SOD (Mn-SOD/SOD2) and extracellular Cu, Zn-SOD (EC-SOD/SOD3). SOD1 is found in all eukaryotic species as a homodimeric 32-kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80-kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin binding multimer of disulfide linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase(CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.

Long Name

Superoxide Dismutase-1

Alternate Names

Cu-Zn SOD, CuZn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble

Gene Symbol

SOD1

Additional SOD1/Cu-Zn SOD Products

Product Documents for SOD1/Cu-Zn SOD Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SOD1/Cu-Zn SOD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SOD1/Cu-Zn SOD Antibody

Customer Reviews for SOD1/Cu-Zn SOD Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • SOD1/Cu-Zn SOD Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Ovary tissue
    Species: Pig
    Verified Customer | Posted 11/17/2016
    SOD1 on porcine whole ovarian homogenate
    Sample Information: Treatment: None. Run on control tissue Controls: Positive Control: None Negative Control: Primary only, secondary only, and secondary with IgG all negative Loading Control: Ponceau S stain Total Protein Loaded: 40 ug/well Electrophoresis: Gel Percentage: Gradient, 4-20% Electrophoresis Conditions: Voltage: 50V then 90V Time: 5 min then 1 hr Membrane Transfer: Method (Submersion/Semi-dry): Semi-dry using iBlot2 system Membrane Type (PVDF/Nitrocellulose): Nitrocellulose Time: 7 min (iBlot2 Protocol 0) Blocking: Blocking Solution: 5% BSA in.2% PBST Time: 1.5 hours Primary Antibody: Dilution: 1:1000 Diluent Buffer: 5% BSA in.2% PBST Incubation Time: overnight Incubation Temperature: 4°C Washing Conditions: Wash Solution:.2% PBST Time and Repetitions: 3X, 10 minutes each Secondary Antibody Manufacturer and Catalog #: Cell Signaling, #7074 Secondary description: goat anti-rabbit Dilution: 1:1000 Diluent Buffer: 5% BSA in.2% PBST Incubation Time: 1 hour Incubation Temperature: room temperature Detection Method: Detection: SignalFire ECL reagent (Cell Signaling) Procedure: ECL on blot 3 min Development Time: 5 min Molecular weight of band(s): 13 kDa
    SOD1/Cu-Zn SOD Antibody NBP1-31204

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