SOX10 Antibody (CL4455) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-57897

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL4455

Format

Azide and BSA Free
View all SOX10 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P56693, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SOX10 Antibody (CL4455) - Azide and BSA Free

SOX10 Antibody (CL4455) - BSA Free

SOX10 Antibody (CL4455) - BSA Free

Analysis in human cerebral cortex and pancreas tissues using NBP3-57814 antibody. Corresponding SOX10 RNA-seq data are presented for the same tissues.
SOX10 Antibody (CL4455) - BSA Free

SOX10 Antibody (CL4455) - BSA Free

Staining of human breast shows moderate to strong nuclear positivity in glandular cells.
SOX10 Antibody (CL4455) - BSA Free

SOX10 Antibody (CL4455) - BSA Free

Staining of human cerebral cortex shows strong nuclear positivity in glial cells (oligodendrocytes).
SOX10 Antibody (CL4455) - BSA Free

SOX10 Antibody (CL4455) - BSA Free

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
SOX10 Antibody (CL4455) - BSA Free

SOX10 Antibody (CL4455) - BSA Free

Staining of human skin shows moderate to strong nuclear positivity in the basal layer of skin epithelial cells.
SOX10 Antibody (CL4455) - BSA Free

SOX10 Antibody (CL4455) - BSA Free

Staining of human squamous epithelium shows strong nuclear positivity in stratum basal cells.
SOX10 Antibody (CL4455) - BSA Free

SOX10 Antibody (CL4455) - BSA Free

Staining of mouse embryo E11 shows strong positivity in the developing trigeminal ganglion.
SOX10 Antibody (CL4455) - BSA Free

SOX10 Antibody (CL4455) - BSA Free

Staining of SK-MEL-30 cells using the Anti-SOX10 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
SOX10 Antibody (CL4455) - BSA Free

SOX10 Antibody (CL4455) - BSA Free

Staining of SK-MEL-30 cells using the Anti-SOX10 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Applications for SOX10 Antibody (CL4455) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX10

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

Long Name

SRY-box transcription factor 10

Alternate Names

DOM, PCWH, SOX-10, WS2E, WS4, WS4C

Gene Symbol

SOX10

Additional SOX10 Products

Product Documents for SOX10 Antibody (CL4455) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SOX10 Antibody (CL4455) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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