SOX4 Antibody

Novus Biologicals | Catalog # NBP3-18643

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
View all SOX4 Primary Antibodies »

Product Specifications

Immunogen

Polyclonal antibody raised in rabbit against human SOX4 (SRY (sex determining region Y)-box 4), using two KLH-conjugated synthetic peptides containing a sequence from the N-terminus and from the C-terminus of the protein, respectively.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for SOX4 Antibody

Western Blot: SOX4 Antibody [NBP3-18643]

Western Blot: SOX4 Antibody [NBP3-18643]

Western Blot: SOX4 Antibody [NBP3-18643] - Figure 4. IP using the antibody directed against SOX4 IP was performed overnight on protein extracts (600 ug) from CT-2A (A) and GL261 (B) cells using 6 ul of the antibody against SOX4. The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 2 shows the result of the IP; the input is shown in lane 1.
Western Blot: SOX4 Antibody [NBP3-18643]

Western Blot: SOX4 Antibody [NBP3-18643]

Western Blot: SOX4 Antibody [NBP3-18643] - Figure 2. Western blot analysis using the antibody directed against SOX4 A. H1299 lung carcinoma cells were transfected with HA-tagged SOX4 (lane 1) or with a HA-tagged truncated SOX4 (395X mutant, lane 2). Whole cell extracts (60 - 100 ug) were analysed by Western blot using the antibody against SOX4 diluted 1:4,000 in TBS-Tween containing 2% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. B. Whole cell extracts of 4 different lung carcinoma cell lines were analysed by Western blot with the antibody against SOX4 as described above. The H522 cells are known to overexpress SOX4. Western blot performed by Montse Sanchez-Cespedes and Sandra Castillo Diez, Catalan Institute of Oncology, Barcelona, Spain.
Western Blot: SOX4 Antibody [NBP3-18643]

Western Blot: SOX4 Antibody [NBP3-18643]

Western Blot: SOX4 Antibody [NBP3-18643] - Figure 3. Western blot analysis using the antibody directed against SOX4 Western blot was performed on Protein extracts (~40 ug) from mouse B16-OVA (A), CT-2A (B), GL261 (C) or B16A (D) cells using the antibody against SOX4. The cells were transfected with a SOX4 specific crRNA (lanes 2) or with a negative control crRNA (lanes 1). The antibody was diluted 1:4,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right. The lower panel shows the WB results with a GAPDH antibody used as loading control. Western blot performed by Anze Godicelj, Dana Farber Cancer Institute, Boston, USA.
ELISA: SOX4 Antibody [NBP3-18643]

ELISA: SOX4 Antibody [NBP3-18643]

ELISA: SOX4 Antibody [NBP3-18643] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human SOX4. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:18,000.

Applications for SOX4 Antibody

Application
Recommended Usage

ELISA

1:100-1:1000

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Immunoprecipitation

6 ul/IP

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

Whole antiserum

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: SOX4

SOX4 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.

Alternate Names

ecotropic viral integration site 16, EVI16, SRY (sex determining region Y)-box 4, SRY-related HMG-box gene 4, transcription factor SOX-4

Gene Symbol

SOX4

UniProt

Q06945 (Human)

Additional SOX4 Products

Product Documents for SOX4 Antibody

Certificate of Analysis

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Product Specific Notices for SOX4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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