SP1 Antibody - BSA Free

Novus Biologicals | Catalog # NB600-233

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SP1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 750 and the C-terminus (residue 785) using the numbering given in entry XP_028606.4 (GeneID 6667).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-55324, NB100-224, NB200-171, NB500-160, NB200-347

Scientific Data Images for SP1 Antibody - BSA Free

Western Blot: SP1 Antibody [NB600-233]

Western Blot: SP1 Antibody [NB600-233]

Western Blot: SP1 Antibody [NB600-233] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233]

Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233]

Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Chromatin Immunoprecipitation: SP1 Antibody [NB600-233] - Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of Sp1 binding within a 250 Kb region of chromosome 6 asdetected using anti-Sp1.
Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233]

Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233]

Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - Human breast carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: SP1 Antibody [NB600-233]

Immunoprecipitation: SP1 Antibody [NB600-233]

Immunoprecipitation: SP1 Antibody [NB600-233] - Detection of Human SP1 on HeLa whole cell lysate using NB600-233. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibody NB600-233. NB600-233 was used at 0.04 mcg/ml for WB.
Immunoprecipitation: SP1 Antibody [NB600-233]

Immunoprecipitation: SP1 Antibody [NB600-233]

Immunoprecipitation: SP1 Antibody [NB600-233] - Detection of human SP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SP1 antibody NB600-233 (lot NB600-233-5) used for IP at 6 ug per reaction. SP1 was also immunoprecipitated by a previous lot of this antibody (NB600-233-4) and another rabbit anti-SP1 antibody. For blotting immunoprecipitated SP1, NB600-233 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for SP1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

4 ug/30 ug lysate

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SP1

Sp1 is one of the first cellular transcription factors to be identified, cloned and characterized for its ability to bind to GC-boxes in regulatory promoter elements. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.

Alternate Names

Sp1 transcription factor, specificity protein 1, transcription factor Sp1, TSFP1

Entrez Gene IDs

6667 (Human)

Gene Symbol

SP1

UniProt

P08047 (Human)

Additional SP1 Products

Product Documents for SP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SP1 Antibody - BSA Free

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-55324, NB100-224, NB200-171, NB500-160, NB200-347

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SP1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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