SPAG9 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-79794

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SPAG9 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 775 and 825 of human JNK/SAPK-Associated Protein using the numbering given in entry NP_003962.3 (GeneID 9043).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPAG9 Antibody - BSA Free

Western Blot: SPAG9 Antibody [NB100-79794]

Western Blot: SPAG9 Antibody [NB100-79794]

Western Blot: SPAG9 Antibody [NB100-79794] - Detection of mouse JLP by western blot. Samples: Whole cell lysate from TCKM (50 ug), Renca (50 ug), and CT26 (50 ug) cells. Antibodies: Affinity purified rabbit anti-JLP antibody NB100-79794 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry: SPAG9 Antibody [NB100-79794]

Immunohistochemistry: SPAG9 Antibody [NB100-79794]

Immunohistochemistry: SPAG9 Antibody [NB100-79794] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-JLP used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: SPAG9 Antibody [NB100-79794]

Western Blot: SPAG9 Antibody [NB100-79794]

Western Blot: SPAG9 Antibody [NB100-79794] - Nuclear extract (NE, 20 ug) from HeLa cells. Antibody used at 0.3 ug/ml.
Western Blot: SPAG9 Antibody [NB100-79794]

Western Blot: SPAG9 Antibody [NB100-79794]

Western Blot: SPAG9 Antibody [NB100-79794] - Detection of Human JLP by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug) and 293T (50ug) cells. Antibodies: Affinity purified rabbit anti-JLP antibody NB100-79794 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: SPAG9 Antibody [NB100-79794]

Immunoprecipitation: SPAG9 Antibody [NB100-79794]

Immunoprecipitation: SPAG9 Antibody [NB100-79794] - Detection of human JLP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JLP antibody NB100-79794 (lot NB100-79794-2) used for IP at 6 ug/mg lysate. JLP was also immunoprecipitated by a previous lot (NB100-79794-1) of this antibody. For blotting immunoprecipitated JLP, NB100-79794 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for SPAG9 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:1000 - 1:10000
Application Notes
For IHC-P, antigen retrieval with citrate buffer pH 6.0 is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SPAG9

Extracellular signals are transduced into cells through mitogen-activated protein kinases. The structural organization of these kinases into specific signaling domains is facilitated by scaffolding proteins involved in closely tethering different kinases so that successive phosphorylation events can occur. The protein encoded by this gene is a scaffolding protein that brings together mitogen-activated protein kinases and their transcription factor targets for the activation of specific signaling pathways. This gene which is abundantly expressed in testicular haploid germ cells encodes a protein that is recognized by sperm-agglutinating antibodies and implicated in infertility.

Alternate Names

Cancer/testis antigen 89, c-Jun NH2-terminal kinase-associated leucine zipper protein, C-Jun-amino-terminal kinase-interacting protein 4, CT89JIP4, FLJ14006, FLJ26141, FLJ34602, HLC4, HLC-6, HSS, Human lung cancer oncogene 6 protein, JLPPHETSYD1FLJ13450, JNK interacting protein, JNK/SAPK-associated protein, JNK-associated leucine-zipper protein, JNK-interacting protein 4, KIAA0516JIP-4, lung cancer oncogene 4, MAPK8IP4, Max-binding protein, MGC117291, MGC14967, MGC74461, Mitogen-activated protein kinase 8-interacting protein 4, PIG6, proliferation-inducing gene 6, Proliferation-inducing protein 6, Protein highly expressed in testis, sperm associated antigen 9, Sperm surface protein, Sperm-associated antigen 9, Sperm-specific protein, Sunday driver 1

Entrez Gene IDs

9043 (Human)

Gene Symbol

SPAG9

UniProt

NP_003962.3 (Human)

Additional SPAG9 Products

Product Documents for SPAG9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SPAG9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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