Src Antibody (7G6M9)

Name: Src Antibody (7G6M9)
Brand: Novus Biologicals
SKU: NBP3-15675
Price: 179 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15675

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7G6M9 expressed in HEK293

View all Src Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Src (P12931). MGSNKSKPKDASQRRRSLEPAENVHGAGGGAFPASQTPSKPASADGHRGPSAAFAPAAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Knockout (KO) Validated Rabbit Src Antibody (7G6M9) (NBP3-15675) is a recombinant monoclonal antibody validated for use in WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Src Antibody (7G6M9)

Immunoprecipitation: Src Antibody (7G6M9) [Src] -

Immunoprecipitation: Src Antibody (7G6M9) [Src] - Immunoprecipitation analysis of 600 ug extracts of Mouse brain using 3 ug Src antibody. Western blot was performed from the immunoprecipitate using Src antibody at a dilution of 1:1000.

Western Blot: Src Antibody (7G6M9) [NBP3-15675]

Western Blot: Src Antibody (7G6M9) [NBP3-15675] - Western blot analysis of extracts of Jurkat cells, using Src antibody (NBP3-15675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Western Blot: Src Antibody (7G6M9) [NBP3-15675]

Western Blot: Src Antibody (7G6M9) [NBP3-15675] - Western blot analysis of extracts of various cell lines, using Src antibody (NBP3-15675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western Blot: Src Antibody (7G6M9) [NBP3-15675]

Western Blot: Src Antibody (7G6M9) [NBP3-15675] - Western blot analysis of extracts from normal (control) and Src knockout (KO) HeLa cells, using Src antibody (NBP3-15675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Immunocytochemistry/ Immunofluorescence: Src Antibody (7G6M9) [Src] -

Immunocytochemistry/ Immunofluorescence: Src Antibody (7G6M9) [Src] - Confocal imaging of NIH/3T3 cells using [KO Validated] Src Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Immunocytochemistry/ Immunofluorescence: Src Antibody (7G6M9) [Src] -

Immunocytochemistry/ Immunofluorescence: Src Antibody (7G6M9) [Src] - Confocal imaging of A549 cells using [KO Validated] Src Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

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Applications for Src Antibody (7G6M9)

Application

Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:100 - 1:400

Immunoprecipitation

0.5μg-4μg antibody for 400μg-600μg extracts of whole cells

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Src

Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3). Mutation of Y529 leads to Scr overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4).

Long Name

v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog

Alternate Names

ASV, c-Src, RSVgp4

Gene Symbol

SRC

Additional Src Products

Product Documents for Src Antibody (7G6M9)

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Product Specific Notices for Src Antibody (7G6M9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.