SSH3 Antibody

Novus Biologicals | Catalog # NB100-60675

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SSH3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 609 and 659 of human slingshot homolog 3 using the numbering given in entry NP_060327.2 (GeneID 54961).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SSH3 Antibody

Western Blot: SSH3 Antibody [NB100-60675]

Western Blot: SSH3 Antibody [NB100-60675]

Western Blot: SSH3 Antibody [NB100-60675] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NB100-60675 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SSH3 was also immunoprecipitated by rabbit anti-SSH3 antibody NB100-60674, which recognizes an upstream epitope.
Immunohistochemistry: SSH3 Antibody [NB100-60675]

Immunohistochemistry: SSH3 Antibody [NB100-60675]

Immunohistochemistry: SSH3 Antibody [NB100-60675] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SSH3 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: SSH3 Antibody [NB100-60675]

Immunohistochemistry-Paraffin: SSH3 Antibody [NB100-60675]

Immunohistochemistry-Paraffin: SSH3 Antibody [NB100-60675] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-SSH3 used at a dilution of 1:250.

Applications for SSH3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SSH3

SSH3, also known as slingshot-3, may function as a phosphatase that activates the actin severing protein, ADF/cofilin to indfluence actin dynamics. There are three members of the slingshot family, SSH1, SSH2, and SSH3.

Alternate Names

EC 3.1.3.16, EC 3.1.3.48, FLJ10928, FLJ20515, FLJ42240, hSSH-3L, protein phosphatase Slingshot homolog 3, slingshot 3, slingshot homolog 3 (Drosophila), SSH3, SSH3L, SSH-3L, SSH-like protein 3

Entrez Gene IDs

54961 (Human)

Gene Symbol

SSH3

UniProt

NP_060327.2 (Human)

Additional SSH3 Products

Product Documents for SSH3 Antibody

Certificate of Analysis

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Product Specific Notices for SSH3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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