STEAP1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-76822
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Porcine (94%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the center of human STEAP1. The immunogen is located within amino acids 130 - 180 of STEAP1. Amino Acid Squence: LHNGTKYKKFPHWLDK
Specificity
This STEAP1 antibody does not cross-react with other STEAP proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit STEAP1 Antibody - BSA Free (NBP1-76822) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for STEAP1 Antibody - BSA Free
Western Blot: STEAP1 Antibody - BSA Free [NBP1-76822] -
Western Blot: STEAP1 Antibody - BSA Free [NBP1-76822] - WB Validation in Mouse Brain Loading: 10 μg of lysate per lane.Antibodies: Steap1 4305, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Exposure: 30 sec Lane 1: 0.5 ug/mL Lane 2: 1 ug/mL
Western Blot: STEAP1 Antibody - BSA Free [NBP1-76822] -
Western Blot: STEAP1 Antibody - BSA Free [NBP1-76822] - WB Validation in Rat Brain Loading: 10 μg of lysate per lane.Antibodies: Steap1 4305, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 30 secLane 1: 0.5 μg/mLLane 2: 1 μg/mL
Immunohistochemistry: STEAP1 Antibody - BSA Free [NBP1-76822] -
Immunohistochemistry: STEAP1 Antibody - BSA Free [NBP1-76822] - Immunohistochemistry of STEAP1 in human spleen tissue with STEAP1 antibody at 2.5 ug/mL.
Immunocytochemistry/ Immunofluorescence: STEAP1 Antibody - BSA Free [NBP1-76822] -
Immunocytochemistry/ Immunofluorescence: STEAP1 Antibody - BSA Free [NBP1-76822] - Immunofluorescence of STEAP1 in human spleen tissue with STEAP1 antibody at 20 ug/mL.Applications for STEAP1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
2.5 ug/ml
Immunohistochemistry-Paraffin
2.5 ug/ml
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: STEAP1
Long Name
Six Transmembrane Epithelial Antigen of the Prostate 1
Alternate Names
PRSS24
Gene Symbol
STEAP1
UniProt
Additional STEAP1 Products
Product Documents for STEAP1 Antibody - BSA Free
Product Specific Notices for STEAP1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for STEAP1 Antibody - BSA Free
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Q: I’m interessted in your STEP1 antibody (NBP1-76822). Could you precise what kind of affinity purified step did you perform to get the antibody ? would you have any further details on ELISA assays ?
A: This antibody was purified by using affinity chromatography using the peptide immunogen. What information are you looking for in ELISA? We recommend a starting dilution of 1:100-1:2000.
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