STIM1 Antibody (5A2) - Azide and BSA Free

Novus Biologicals | Catalog # H00006786-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5A2

Format

Azide and BSA Free
View all STIM1 Primary Antibodies »

Product Specifications

Immunogen

STIM1 (AAH21300, 24 a.a. ~ 685 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGAVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQSSVRQRLTEPQHGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQMSRAADEALNAMTSNGSHRLIEGVHPGSLVEKLPDSPALAKKALLALNHGLDKAHSLMELSPSAPPGGSPHLDSSRSHSPSSPDPDTPSPVGDSRALQASRNTRIPHLAGKKAVAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23076152). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

STIM1 (5A2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for STIM1 Antibody (5A2) - Azide and BSA Free

Western Blot: STIM1 Antibody (5A2) [H00006786-M01]

Western Blot: STIM1 Antibody (5A2) [H00006786-M01]

Western Blot: STIM1 Antibody (5A2) [H00006786-M01] - STIM1 monoclonal antibody (M01), clone 5A2. Analysis of STIM1 expression in human liver.
Immunocytochemistry/ Immunofluorescence: STIM1 Antibody (5A2) [H00006786-M01]

Immunocytochemistry/ Immunofluorescence: STIM1 Antibody (5A2) [H00006786-M01]

Immunocytochemistry/Immunofluorescence: STIM1 Antibody (5A2) [H00006786-M01] - Analysis of monoclonal antibody to STIM1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: STIM1 Antibody (5A2) [H00006786-M01]

Immunohistochemistry-Paraffin: STIM1 Antibody (5A2) [H00006786-M01]

Immunohistochemistry-Paraffin: STIM1 Antibody (5A2) [H00006786-M01] - Analysis of monoclonal antibody to STIM1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B. Antibody concentration 3 ug/ml.
Western Blot: STIM1 Antibody (5A2) [H00006786-M01]

Western Blot: STIM1 Antibody (5A2) [H00006786-M01]

Western Blot: STIM1 Antibody (5A2) [H00006786-M01] - STIM1 expression in HeLa ( Cat # L013V1 ).
Western Blot: STIM1 Antibody (5A2) [H00006786-M01]

Western Blot: STIM1 Antibody (5A2) [H00006786-M01]

Western Blot: STIM1 Antibody (5A2) [H00006786-M01] - STIM1 monoclonal antibody (M01), clone 5A2. Western Blot analysis of STIM1 expression in K-562 ( Cat # L009V1 ).
ELISA: STIM1 Antibody (5A2) [H00006786-M01]

ELISA: STIM1 Antibody (5A2) [H00006786-M01]

ELISA: STIM1 Antibody (5A2) [H00006786-M01] - Detection limit for recombinant GST tagged STIM1 is approximately 0.3ng/ml as a capture antibody.

Applications for STIM1 Antibody (5A2) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. IT has been used for IF, IHC-P, and ELISA. IP usage reported in scientific literature (PMID: 24845346).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STIM1

This gene encodes a type 1 transmembrane protein of unknown function. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene.

Long Name

Stromal Interaction Molecule 1

Alternate Names

GOK

Entrez Gene IDs

6786 (Human)

Gene Symbol

STIM1

OMIM

605921 (Human)

UniProt

AAH21300 (Human)

Additional STIM1 Products

Product Documents for STIM1 Antibody (5A2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for STIM1 Antibody (5A2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Ion Channels and Regulators

Citations for STIM1 Antibody (5A2) - Azide and BSA Free

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Protocols

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FAQs

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