stonin-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90658

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all stonin-2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RTPSVTEAPPWRATNPFLNETLQDVQPSPINPFSAFFEEQERRSQNSSISSTTGKSQRDSLIVIYQDAISFDDSSKTQSHSDAVEKLKQLQIDDPDHFGSATLPDDDPVAWIELDAHPPGSARSQPRDGWPMMLRIPEK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for stonin-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658]

Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658]

Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658] - Analysis in human cerebral cortex and liver tissues using NBP1-90658 antibody. Corresponding STON2 RNA-seq data are presented for the same tissues.
Western Blot: stonin-2 Antibody [NBP1-90658]

Western Blot: stonin-2 Antibody [NBP1-90658]

Western Blot: stonin-2 Antibody [NBP1-90658] - Analysis in human cell line K562.
Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658]

Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658]

Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658] - Staining of human duodenum shows moderate positivity in apical membrane in glandular cells.
Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658]

Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658]

Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658]

Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658]

Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658]

Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658]

Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658] - Staining of human liver shows no positivity in hepatocytes as expected.
Simple Western: stonin-2 Antibody [NBP1-90658]

Simple Western: stonin-2 Antibody [NBP1-90658]

Simple Western: stonin-2 Antibody [NBP1-90658] - Simple Western lane view shows a specific band for stonin-2 in 0.2 mg/ml of HEL lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.
Simple Western: stonin-2 Antibody [NBP1-90658]

Simple Western: stonin-2 Antibody [NBP1-90658]

Simple Western: stonin-2 Antibody [NBP1-90658] - Electropherogram image of the corresponding Simple Western lane view. stonin-2 antibody was used at 1:5 dilution on HEL lysate(s) respectively.
stonin-2 Antibody - BSA Free Western Blot: stonin-2 Antibody - BSA Free [NBP1-90658]

Western Blot: stonin-2 Antibody - BSA Free [NBP1-90658]

Analysis in human cell line K562.
stonin-2 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: stonin-2 Antibody [NBP1-90658]

Immunocytochemistry/ Immunofluorescence: stonin-2 Antibody [NBP1-90658]

Staining of human cell line A-431 shows positivity in nucleoli & cytoplasm.

Applications for stonin-2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:5

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HEL lysates, separated by Size, antibody dilution of 1:5

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: stonin-2

Alternate Names

STN2, STNB, STNB2, stoned B homolog 2, stonin 2, stonin-2

Gene Symbol

STON2

Additional stonin-2 Products

Product Documents for stonin-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for stonin-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for stonin-2 Antibody - BSA Free

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Protocols

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FAQs

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