STX18 Antibody (2E5) - Azide and BSA Free

Novus Biologicals | Catalog # H00053407-M13

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E5

Format

Azide and BSA Free
View all STX18 Primary Antibodies »

Product Specifications

Immunogen

STX18 (NP_058626, 101 a.a. ~ 199 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QIFMRTCSEAIQQLRTEAHKEIHSQQVKEHRTAVLDFIEDYLKRVCKLYSEQRAIRVKRVVDKKRLSKLEPEPNTKTRESTSSEKVSQSPSKDSEENPA

Specificity

Reacts with syntaxin 18.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for STX18 Antibody (2E5) - Azide and BSA Free

Western Blot: STX18 Antibody (2E5) [H00053407-M13]

Western Blot: STX18 Antibody (2E5) [H00053407-M13]

Western Blot: STX18 Antibody (2E5) [H00053407-M13] - Analysis of STX18 expression in PC-12 (Cat # L012V1).
Immunohistochemistry-Paraffin: STX18 Antibody (2E5) [H00053407-M13]

Immunohistochemistry-Paraffin: STX18 Antibody (2E5) [H00053407-M13]

Immunohistochemistry-Paraffin: STX18 Antibody (2E5) [H00053407-M13] - Analysis of monoclonal antibody to STX18 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml
Western Blot: STX18 Antibody (2E5) [H00053407-M13]

Western Blot: STX18 Antibody (2E5) [H00053407-M13]

Western Blot: STX18 Antibody (2E5) [H00053407-M13] - Analysis of STX18 expression in Raw 264.7 (Cat # L024V1).
Sandwich ELISA: STX18 Antibody (2E5) [H00053407-M13] - Detection limit for recombinant GST tagged STX18 is 0.1 ng/ml as a capture antibody.

Applications for STX18 Antibody (2E5) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against cell lysate in western blot and recombinant protein in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STX18

Syntaxin that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER

Alternate Names

Cell growth-inhibiting gene 9 protein, DKFZp686O15149, syntaxin 18, syntaxin-18, Ufe1

Entrez Gene IDs

53407 (Human)

Gene Symbol

STX18

UniProt

NP_058626 (Human)

Additional STX18 Products

Product Documents for STX18 Antibody (2E5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for STX18 Antibody (2E5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for STX18 Antibody (2E5) - Azide and BSA Free

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  • Q: Could you please tell me the recommended dilution ratio for WB for this product or at least a starting point that you recommend for H00053407-M13?

    A:

    H00053407-M13 is manufactured by Abnova and their product page just points you towards their general protocol and does not list and specifics.
    http://www.abnova.com/products/products_detail.asp?catalog_id=H00053407-M13#p00
    According to their general protocol, their mouse monoclonal antibodies (Mouse Ig) are recommended at a concentration of 1-5 ug/ml.
    See table 2: http://www.abnova.com/protocol_pdf/Western_Blot.pdf

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