SUCLA2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33015

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (92%), Chicken (90%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SUCLA2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SUCLA2. The exact sequence is proprietary.

Reactivity Notes

Rat (88%), Zebrafish (81%), Xenopus laevis (85%).

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SUCLA2 Antibody - BSA Free

Western Blot: SUCLA2 Antibody [NBP1-33015]

Western Blot: SUCLA2 Antibody [NBP1-33015]

Western Blot: SUCLA2 Antibody [NBP1-33015] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 10% SDS PAGE diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SUCLA2 Antibody [NBP1-33015]

Immunocytochemistry/ Immunofluorescence: SUCLA2 Antibody [NBP1-33015]

Immunocytochemistry/Immunofluorescence: SUCLA2 Antibody [NBP1-33015] - SUCLA2 antibody [N2C3] detects SUCLA2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: SUCLA2 protein stained by SUCLA2 antibody [N2C3] diluted at 1:500.Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP1-33015]

Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP1-33015]

Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP1-33015] - Mouse stomach. SUCLA2 antibody [N2C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: SUCLA2 Antibody [NBP1-33015]

Western Blot: SUCLA2 Antibody [NBP1-33015]

Western Blot: SUCLA2 Antibody [NBP1-33015] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:20000.
Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP1-33015]

Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP1-33015]

Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP1-33015] - Cal27 xenograft, using SUCLA2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: SUCLA2 Antibody [NBP1-33015]

Western Blot: SUCLA2 Antibody [NBP1-33015]

Western Blot: SUCLA2 Antibody [NBP1-33015] - Wild-type (WT) and SUCLA2 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLA2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SUCLA2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
See Simple Western Antibody Database for Simple Western validation

Reviewed Applications

Read 1 review rated 5 using NBP1-33015 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SUCLA2

Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6. (provided by RefSeq)

Alternate Names

A-BETA, ATP-specific succinyl-CoA synthetase subunit beta, beta subunit, EC 6.2.1, EC 6.2.1.5, mitochondrial succinyl-CoA ligase [ADP-forming] subunit beta, MTDPS5, Renal carcinoma antigen NY-REN-39, succinate-CoA ligase, ADP-forming, beta subunit, succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial

Gene Symbol

SUCLA2

Additional SUCLA2 Products

Product Documents for SUCLA2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SUCLA2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for SUCLA2 Antibody - BSA Free

Customer Reviews for SUCLA2 Antibody - BSA Free (1)

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  • SUCLA2 Antibody
    Name: zhenqi zhou
    Application: Western Blot
    Sample Tested: C2C12 mouse myoblast cell line and Mouse skeletal muscle homogenate
    Species: Mouse
    Verified Customer | Posted 03/12/2019
    SUCLA2 Antibody - BSA Free NBP1-33015

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