SUN1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87396

Novus Biologicals
Loading...

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
Loading...

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QLLPTVEHLQLELDQLKSELSSWRHVKTGCETVDAVQERVDVQVREMVKLLFSEDQQGGSLEQLLQRFSSQFVSKGDLQTMLRDLQLQILRNVTHHVSVTKQLPTSEAVVSAVSEAGASGITEAQARAIVNS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUN1 Antibody - BSA Free

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Analysis in human skin and bone marrow tissues. Corresponding SUN1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human fallopian tube, kidney, skin and testis using Anti-SUN1 antibody NBP1-87396 (A) shows similar protein distribution across tissues to independent antibody NBP1-87395 (B).
Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396]

Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396]

SUN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87396-img0027.jpg
Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human testis shows strong positivity in nuclear membrane in Leydig cells.
Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396]

Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396]

Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87396] - Staining of A431 cells at 4ug/ml against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (red) and DAPI (blue), respectively.
Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396]

Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396]

Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87396] - Staining of human cell line A-431 shows positivity in nuclear membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human bone marrow shows low positivity in hematopoietic cells as expected.
Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human Fallopian tube shows moderate positivity in nuclear membrane in glandular cells.
Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human kidney shows strong positivity in nuclear membrane in cells in glomeruli.
Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human skin shows strong positivity in nuclear membrane in squamous epithelial cells.
SUN1 Antibody

Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396] -

Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396] - Examples of nuclear morphology defects in (A) MCF7 & (B) U2OS cell lines 24 h PI with the dose of 8 Gy of gamma -rays. (A) 1—An anaphase bridge linking the separating daughter nuclei, SUN1 (red), nesprin1 (green), 2—Convoluted nucleus with blisters (arrows), LA/C (green), emerin (red). 3—Fragmented nucleus represented by 3 large & numerous small micronuclei. SUN2 (red), nesprin-1 (green). (B) 1— A giant nucleus with small chromatin fragments & micronuclei (MN, arrows), SUN1 (red), nesprin-1 (green). 2—Nucleus with micronuclei, emerin (red), lamin A/C (green), arrows indicate MN containing emerin & heterochromatin (HC), 3—Convoluted nucleus with blisters (arrows), emerin (red), LA/C (green). 4—Fragmented nucleus, emerin (red), LA/C (green). 5—Honeycombed nucleus, emerin (red), LA/C (green), thick arrow shows interrupted NE enabling chromatin to leak out of the nucleus, reinforced layer of LA/C (green) & emerin (red), (thin arrows). Size bar indicates µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32316379), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SUN1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Simple Western

1:20
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 113 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SUN1

This gene is a member of the unc-84 homolog family and encodes a nuclear nuclear envelope protein with an Unc84 (SUN) domain. The protein is involved in nuclear anchorage and migration. Several alternatively spliced transcript variants of this gene have been described; however, the full-length nature of some of these variants has not been determined.

Alternate Names

KIAA0810UNC84AFLJ12407, MGC176649, Protein unc-84 homolog A, Sad1 and UNC84 domain containing 1, Sad1 unc-84 domain protein 1, Sad1/unc-84 protein-like 1, SUN domain-containing protein 1, unc-84 homolog A, unc-84 homolog A (C. elegans)

Entrez Gene IDs

23353 (Human)

Gene Symbol

SUN1

UniProt

Additional SUN1 Products

Product Documents for SUN1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SUN1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SUN1 Antibody - BSA Free

Customer Reviews for SUN1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review SUN1 Antibody - BSA Free and earn rewards!

Have you used SUN1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...