TAF15 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13410

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: DQQSGYDQHQGSYDEQSNYDQQHDSYSQNQQSYHSQRENYSHHTQDDRRDVSRYGEDNRGYGGSQGGG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TAF15 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TAF15 Antibody [NBP2-13410]

Immunocytochemistry/ Immunofluorescence: TAF15 Antibody [NBP2-13410]

Immunocytochemistry/Immunofluorescence: TAF15 Antibody [NBP2-13410] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: TAF15 Antibody [NBP2-13410]

Immunohistochemistry-Paraffin: TAF15 Antibody [NBP2-13410]

Immunohistochemistry-Paraffin: TAF15 Antibody [NBP2-13410] - Staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: TAF15 Antibody [NBP2-13410]

Immunohistochemistry-Paraffin: TAF15 Antibody [NBP2-13410]

Immunohistochemistry-Paraffin: TAF15 Antibody [NBP2-13410] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: TAF15 Antibody [NBP2-13410]

Immunohistochemistry-Paraffin: TAF15 Antibody [NBP2-13410]

Immunohistochemistry-Paraffin: TAF15 Antibody [NBP2-13410] - Staining of human testis shows high expression.
TAF15 Antibody - BSA Free Immunohistochemistry: TAF15 Antibody - BSA Free [NBP2-13410]

Immunohistochemistry: TAF15 Antibody - BSA Free [NBP2-13410]

Analysis in human testis and pancreas tissues using Anti-TAF15 antibody. Corresponding TAF15 RNA-seq data are presented for the same tissues.

Applications for TAF15 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TAF15

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.

Alternate Names

68kDa, hTAFII68, Npl3, RBP56TAFII68, RNA-binding protein 56, TAF(II)68, TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, TAF2NRBP56/CSMF fusion, TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD(RNA-binding protein 56), TATA box-binding protein-associated factor 2N (RNA-binding protein 56), TATA-binding protein-associated factor 2N, TBP-associated factor 15,68 kDa TATA-binding protein-associated factor

Gene Symbol

TAF15

Additional TAF15 Products

Product Documents for TAF15 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TAF15 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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