TICRR Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-41283

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Antibody was raised against a 19 amino acid peptide near the carboxy terminus of human TICRR. The immunogen is located within amino acids 1840 - 1890 of TICRR. Amino Acid Squence: FQGKTPSSQSKDPRDEDVD

Specificity

TICRR antibody is human specific. At least two isoforms of TICRR are known to exist.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TICRR Antibody - BSA Free

Western Blot: TICRR AntibodyBSA Free [NBP2-41283]

Western Blot: TICRR AntibodyBSA Free [NBP2-41283]

Western Blot: TICRR Antibody [NBP2-41283] - Western blot analysis of TICRR in human small intestine tissue lysate with TICRR antibody at 1 ug/ml.
TICRR Antibody - BSA Free

Immunohistochemistry: TICRR Antibody - BSA Free [NBP2-41283] -

Immunohistochemistry: TICRR Antibody - BSA Free [NBP2-41283] - Immunohistochemistry of TICRR in human small intestine tissue with TICRR antibody at 5 ug/mL.
TICRR Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TICRR Antibody - BSA Free [NBP2-41283] -

Immunocytochemistry/ Immunofluorescence: TICRR Antibody - BSA Free [NBP2-41283] - Immunofluorescence of TICRR in human small intestine tissue with TICRR antibody at 20 ug/mL.
TICRR Antibody - BSA Free

Western Blot: TICRR Antibody - BSA Free [NBP2-41283] -

Knockdown of TICRR inhibited cell proliferation. (A)TICRR expression was inhibited in MCF7 cells using siRNA and shRNA. Efficiency of TICRR knockdown evaluated by RT-qPCR. Data were mean +/- SEM, N = 3 biological replicates. (B) Relative proliferation in control and TICRR-knockdown MCF7 cells after transfection for 48 h. Western blot (left) was performed. The proliferation in control cells was set at 1. *P < 0.05, two-tailed Student's t-test. Data were mean +/- SEM, N = 3 biological replicates. (C,D) Growth curves of TICRR-knockdown cells and control in MCF7 (C) and HCC1806 (D) cell lines. western blot (left) was performed. Cell viability at 0 h was set at 1. *P < 0.05, **p < 0.01, ****p < 1.0 × 10−4, two-way ANOVA test. Results were displayed as means +/- SEM, N = 3 biological replicates. (E,F) Knockdown of TICRR inhibited the colony formation in MCF7 (E) and HCC1806 (F) cells examined by plate colony formation assay. Representative colonies pictures were shown on the left and percentage of colony number were on the right. *P < 0.05, two-tailed Student's t-test. Results were displayed as means +/- SEM, N = 3 biological replicates. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31275851), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TICRR Antibody - BSA Free

Western Blot: TICRR Antibody - BSA Free [NBP2-41283] -

Knockdown of TICRR inhibited cell proliferation. (A)TICRR expression was inhibited in MCF7 cells using siRNA and shRNA. Efficiency of TICRR knockdown evaluated by RT-qPCR. Data were mean +/- SEM, N = 3 biological replicates. (B) Relative proliferation in control and TICRR-knockdown MCF7 cells after transfection for 48 h. Western blot (left) was performed. The proliferation in control cells was set at 1. *P < 0.05, two-tailed Student's t-test. Data were mean +/- SEM, N = 3 biological replicates. (C,D) Growth curves of TICRR-knockdown cells and control in MCF7 (C) and HCC1806 (D) cell lines. western blot (left) was performed. Cell viability at 0 h was set at 1. *P < 0.05, **p < 0.01, ****p < 1.0 × 10−4, two-way ANOVA test. Results were displayed as means +/- SEM, N = 3 biological replicates. (E,F) Knockdown of TICRR inhibited the colony formation in MCF7 (E) and HCC1806 (F) cells examined by plate colony formation assay. Representative colonies pictures were shown on the left and percentage of colony number were on the right. *P < 0.05, two-tailed Student's t-test. Results were displayed as means +/- SEM, N = 3 biological replicates. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31275851), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TICRR Antibody - BSA Free

Western Blot: TICRR Antibody - BSA Free [NBP2-41283] -

Knockdown of TICRR inhibited cell proliferation. (A)TICRR expression was inhibited in MCF7 cells using siRNA and shRNA. Efficiency of TICRR knockdown evaluated by RT-qPCR. Data were mean +/- SEM, N = 3 biological replicates. (B) Relative proliferation in control and TICRR-knockdown MCF7 cells after transfection for 48 h. Western blot (left) was performed. The proliferation in control cells was set at 1. *P < 0.05, two-tailed Student's t-test. Data were mean +/- SEM, N = 3 biological replicates. (C,D) Growth curves of TICRR-knockdown cells and control in MCF7 (C) and HCC1806 (D) cell lines. western blot (left) was performed. Cell viability at 0 h was set at 1. *P < 0.05, **p < 0.01, ****p < 1.0 × 10−4, two-way ANOVA test. Results were displayed as means +/- SEM, N = 3 biological replicates. (E,F) Knockdown of TICRR inhibited the colony formation in MCF7 (E) and HCC1806 (F) cells examined by plate colony formation assay. Representative colonies pictures were shown on the left and percentage of colony number were on the right. *P < 0.05, two-tailed Student's t-test. Results were displayed as means +/- SEM, N = 3 biological replicates. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31275851), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TICRR Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TICRR

Alternate Names

C15orf42, Chromosome 15 Open Reading Frame 42, SLD3, SLD3 Homolog, SLD3 Homolog (S. Cerevisiae), TOPBP1-Interacting Checkpoint And Replication Regulator, TOPBP1-Interacting Replication-Stimulating Protein, TopBP1-Interacting, Replication-Stimulating Protein, Treslin

Gene Symbol

TICRR

Additional TICRR Products

Product Documents for TICRR Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TICRR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TICRR Antibody - BSA Free

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Protocols

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