TIF1 alpha Antibody (2F2) - Azide and BSA Free

Novus Biologicals | Catalog # H00008805-M01

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 2F2

Format

Azide and BSA Free
View all TIF1 alpha Primary Antibodies »

Product Specifications

Immunogen

TRIM24 (AAH28689, 432 a.a. ~ 569 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PQMPKQNPVVEQNSQPPSGLSSNQLSKFPTQISLAQLRLQHMQQQVMAQRQQVQRRPAPVGLPNPRMQGPIQQPSISHQQPPPRLINFQNHSPKPNGPVLPPHPQQLRYPPNQNIPRQAIKPNPLQMAFLAQQAIKQW

Specificity

TRIM24 - tripartite motif-containing 24

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TIF1 alpha Antibody (2F2) - Azide and BSA Free

Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01]

Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01]

Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01] - TRIM24 monoclonal antibody (M01), clone 2F2. Analysis of TRIM24 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: TIF1 alpha Antibody (2F2) [H00008805-M01]

Immunocytochemistry/ Immunofluorescence: TIF1 alpha Antibody (2F2) [H00008805-M01]

Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of monoclonal antibody to TRIM24 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: TIF1 alpha Antibody (2F2) [H00008805-M01]

Immunohistochemistry-Paraffin: TIF1 alpha Antibody (2F2) [H00008805-M01]

Immunohistochemistry-Paraffin: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of monoclonal antibody to TRIM24 on formalin-fixed paraffin-embedded human testis. Antibody concentration 6 ug/ml.
Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01]

Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01]

Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01] - TRIM24 monoclonal antibody (M01), clone 2F2 Analysis of TRIM24 expression in IMR-32.
Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01]

Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01]

Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of TRIM24 expression in transfected 293T cell line by TRIM24 monoclonal antibody (M01), clone 2F2.Lane 1: TRIM24 transfected lysate(116.8 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: TIF1 alpha Antibody (2F2) [H00008805-M01]

Immunoprecipitation: TIF1 alpha Antibody (2F2) [H00008805-M01]

Immunoprecipitation: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of TRIM24 transfected lysate using anti-TRIM24 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TRIM24 MaxPab rabbit polyclonal antibody.

Applications for TIF1 alpha Antibody (2F2) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TIF1 alpha

The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Long Name

Transcription intermediary factor 1-alpha

Alternate Names

RNF82, TIF1, TIF1-alpha, TIF1A, TRIM24

Entrez Gene IDs

8805 (Human)

Gene Symbol

TRIM24

OMIM

188550 (Human)

UniProt

AAH28689 (Human)

Additional TIF1 alpha Products

Product Documents for TIF1 alpha Antibody (2F2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TIF1 alpha Antibody (2F2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TIF1 alpha Antibody (2F2) - Azide and BSA Free

Customer Reviews for TIF1 alpha Antibody (2F2) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review TIF1 alpha Antibody (2F2) - Azide and BSA Free and earn rewards!

Have you used TIF1 alpha Antibody (2F2) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies