TOR/mTOR Antibody (6611) - Azide and BSA Free

Name: TOR/mTOR Antibody (6611) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-43658
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-43658

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%), Sheep (98%), Zebrafish (90%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 6611

Format

Azide and BSA Free

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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TOR/mTOR. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Theoretical MW

289 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse TOR/mTOR Antibody (6611) - Azide and BSA Free (NBP2-43658) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TOR/mTOR Antibody (6611) - Azide and BSA Free

Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658]

Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658] - Analysis of A Jurkat whole cell lysate/extract B Raji C NCI-H929 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [6611] dilution: 1:1000.

Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658]

Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [6611] dilution: 1:500

TOR/mTOR Antibody (6611) [NBP2-43658] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mTOR stained by mTOR antibody [6611] diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT6611] (NBP2-43658) diluted at 1:500.

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Applications for TOR/mTOR Antibody (6611) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TOR

The mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase which helps to mediate many cellular processes. Specifically, mTOR is invloved in the cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex, making mTOR antibodies useful tools for a number of important research areas.

Long Name

Mammalian Target of Rapamycin

Alternate Names

FRAP1, FRAP2, MTOR, RAFT1

Gene Symbol

MTOR

Additional TOR Products

Product Documents for TOR/mTOR Antibody (6611) - Azide and BSA Free

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Product Specific Notices for TOR/mTOR Antibody (6611) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.