TRBC1 Antibody (38C2) - Chimeric - BSA Free

Novus Biologicals | Catalog # NBP3-43577

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 38C2

Format

BSA Free
View all TRBC1 Primary Antibodies »

Product Specifications

Immunogen

The original antibody was generated by immunizing mice with TCRbetaF1 protein.

Specificity

The antibody is specific for TCRbetaF1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Scientific Data Images for TRBC1 Antibody (38C2) - Chimeric - BSA Free

TRBC1 Antibody (38C2) - Chimeric - BSA Free

Western Blot: TRBC1 Antibody (38C2) - Chimeric - BSA Free [NBP3-43577] -

Western Blot: TRBC1 Antibody (38C2) - Chimeric - BSA Free [NBP3-43577] - Jurkat (A) and Molt4 (B) cells lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with NBP3-43577 at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.
TRBC1 Antibody (38C2) - Chimeric - BSA Free

Immunocytochemistry /Immunofluorescence: TRBC1 Antibody (38C2) - Chimeric - BSA Free [NBP3-43577] -

Immunocytochemistry /Immunofluorescence: TRBC1 Antibody (38C2) - Chimeric - BSA Free [NBP3-43577] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips stained with NBP3-43577 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43577, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.

Applications for TRBC1 Antibody (38C2) - Chimeric - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRBC1

Predicted to enable antigen binding activity and immunoglobulin receptor binding activity. Predicted to be involved in several processes, including activation of immune response; defense response to other organism; and phagocytosis. Predicted to be located in plasma membrane. Predicted to be part of immunoglobulin complex, circulating. Predicted to be active in external side of plasma membrane.

Alternate Names

BV05S1J2.2, TCRBC1, TRBC1 T cell receptor beta constant 1

Gene Symbol

TRBC1

Additional TRBC1 Products

Product Documents for TRBC1 Antibody (38C2) - Chimeric - BSA Free

Certificate of Analysis

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Product Specific Notices for TRBC1 Antibody (38C2) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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