TRNT1 Antibody - BSA Free

Western Blot: TRNT1 Antibody - BSA Free [NBP1-86589]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Western Blot: TRNT1 Antibody - BSA Free [NBP1-86589] -

PK11195 may boost TSPO expression but cannot restore siTRNT1-mediated reduction in inflammatory cytokine production. To examine the role of TSPO in macrophage inflammatory responses, RAW264.7 cells were transfected with siCtrl (siC) or siTRNT1 (siT) for 2 days before co-treatment with 10nM PK11195 and either LPS or Poly (I:C). TRNT1 and TSPO protein levels in each condition were assessed via immunoblotting, with the representative blot shown on the top and quantified data on the bottom (A). All siC and siT samples shown were loaded onto separate Stain-Free gels and presented and cropped as one image. The band intensity was normalized to the total protein levels in each of their respective lanes within their respective gel using the Bio-Rad Stain-Free Application. Inflammatory cytokine expression (IL-1 beta (B), IL-6 (C), TNF (D), IFN-beta (E)) was evaluated via ELISA. mPTP activity was assessed using the Image-IT™ LIVE Mitochondrial Transition Pore Assay Kit (F). Data represents mean +/- SEM of five individual experiments (*p < 0.05, **p < 0.01, and ***p < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41019077), licensed under a CC-BY license. Not internally tested by Novus Biologicals.