TRPS1 Antibody (MSVA-512R)

Novus Biologicals | Catalog # NBP3-57269

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-512R
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Product Specifications

Immunogen

Recombinant fragment (around aa900-1100) of human TRPS1 protein (exact sequence is proprietary) Swissprot Q9UHF7

Localization

Nucleus

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Breast: A strong TRPS1 staining should be seen in luminal breast epithelial cells. (For low-level TRPS1 detection: Testis: A weak nuclear TRPS1 staining should be seen in spermatogonia.).

Negative Control
Negitve control:Colon: Nuclear TRPS1 staining should be absent in epithelial cells.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TRPS1 Antibody (MSVA-512R)

TRPS1 Antibody (MSVA-512R)

Immunohistochemistry-Paraffin:TRPS1 Antibody (MSVA-512R)NBP3-57269

Invasive breast cancer of no special type NST with intense nuclear TRPS1 positivity Dilution 1 600
TRPS1 Antibody (MSVA-512R)

Immunohistochemistry-Paraffin:TRPS1 Antibody (MSVA-512R)NBP3-57269

Intense nuclear TRPS1 staining of luminal but not basal breast epithelial cells Dilution 1 20
TRPS1 Antibody (MSVA-512R)

Immunohistochemistry-Paraffin:TRPS1 Antibody (MSVA-512R)NBP3-57269

Appendical epithelial cells are TRPS1 negative but nuclear TRPS1 positivity occurs in stromal cells Dilution 1 20

Applications for TRPS1 Antibody (MSVA-512R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TRPS1

The TRPS1 gene encodes a transcription factor that represses GATA-regulated genes and binds to a dynein light chain protein. Binding of the encoded protein to the dynein light chain protein affects binding to GATA consensus sequences and suppresses its transcr

Long Name

Tricho-rhino-phalangeal Syndrome Type I Protein

Alternate Names

GC79

Gene Symbol

TRPS1

Additional TRPS1 Products

Product Documents for TRPS1 Antibody (MSVA-512R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TRPS1 Antibody (MSVA-512R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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