Tryptase alpha/beta 1 Antibody (SC68-07)

Novus Biologicals | Catalog # NBP2-67608

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SC68-07 expressed in HEK293
View all Tryptase alpha/beta-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human Tryptase alpha/TPS1 aa 10-275 / 275. (SwissProt: Q15661 Human; SwissProt: Q02844 Mouse; SwissProt: P27435 Rat)

Localization

Secreted.

Specificity

Due to sequence identity this antibody will likely cross react with tryptase beta 2.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Tryptase alpha/beta 1 Antibody (SC68-07)

Western Blot: Tryptase alpha/beta 1 Antibody (SC68-07) [NBP2-67608]

Western Blot: Tryptase alpha/beta 1 Antibody (SC68-07) [NBP2-67608]

Western Blot: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry-Paraffin: Tryptase alpha/beta 1 Antibody (SC68-07) [NBP2-67608]

Immunohistochemistry-Paraffin: Tryptase alpha/beta 1 Antibody (SC68-07) [NBP2-67608]

Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human lung tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: Tryptase alpha/beta 1 Antibody (SC68-07) [NBP2-67608]

Immunohistochemistry-Paraffin: Tryptase alpha/beta 1 Antibody (SC68-07) [NBP2-67608]

Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human tonsil tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: Tryptase alpha/beta 1 Antibody (SC68-07) [NBP2-67608]

Immunohistochemistry-Paraffin: Tryptase alpha/beta 1 Antibody (SC68-07) [NBP2-67608]

Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human small intestine tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: Tryptase alpha/beta 1 Antibody (SC68-07) [NBP2-67608]

Immunohistochemistry-Paraffin: Tryptase alpha/beta 1 Antibody (SC68-07) [NBP2-67608]

Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human prostate carcinoma tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for Tryptase alpha/beta 1 Antibody (SC68-07)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Multiplex Immunofluorescence

1:3000-1:5000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tryptase alpha/beta-1

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

Alternate Names

TPS2, TPSB2, Tryptase beta-2, Tryptase beta2, Tryptase-C

Gene Symbol

TPSAB1

UniProt

Q15661 (Human)

Additional Tryptase alpha/beta-1 Products

Product Documents for Tryptase alpha/beta 1 Antibody (SC68-07)

Certificate of Analysis

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Product Specific Notices for Tryptase alpha/beta 1 Antibody (SC68-07)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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