TSFM Antibody (23GB4710)

Novus Biologicals | Catalog # NBP3-51300

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB4710
View all TSFM Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human TSFM (Uniprot: P43897)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TSFM Antibody (23GB4710)

TSFM Antibody (23GB4710)

Immunocytochemistry/ Immunofluorescence: TSFM Antibody (23GB4710)[NBP3-51300] -

TSFM antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: TSFM shRNA knockdown (KD). Nuclei were stained blue with DAPI; TSFM was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
TSFM Antibody (23GB4710)

Immunocytochemistry/ Immunofluorescence: TSFM Antibody (23GB4710)[NBP3-51300] -

Immunocytochemical staining of HeLa cells with TSFM antibody (1:1,000). Nuclei were stained blue with DAPI; TSFM was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
TSFM Antibody (23GB4710)

Flow Cytometry: TSFM Antibody (23GB4710)[NBP3-51300] -

Validation of TSFM knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with TSFM antibody (1:2,000) and analyzed using BD flow cytometer.
TSFM Antibody (23GB4710)

Flow Cytometry: TSFM Antibody (23GB4710)[NBP3-51300] -

Flow cytometric analysis of TSFMexpression in HeLa cells using TSFM antibody (1:2,000). Green, isotype control; red, TSFM.
TSFM Antibody (23GB4710)

Western Blot: TSFM Antibody (23GB4710)[NBP3-51300] -

Western blotting analysis using TSFM antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for TSFM Antibody (23GB4710)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TSFM

Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes. The TSFM gene encodes a mitochondrial translation elongation factor (Smeitink et al., 2006 [PubMed 17033963]).[supplied by OMIM]

Alternate Names

COXPD3, EFTS, EF-Ts, EFTSMT, EF-TsMt, mitochondrial, mitochondrial elongation factor Ts, Ts translation elongation factor, mitochondrial

Gene Symbol

TSFM

Additional TSFM Products

Product Documents for TSFM Antibody (23GB4710)

Certificate of Analysis

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Product Specific Notices for TSFM Antibody (23GB4710)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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