U1A Antibody (3F9-1F7) - Azide and BSA Free

Novus Biologicals | Catalog # H00006626-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3F9-1F7

Format

Azide and BSA Free
View all U1A Primary Antibodies »

Product Specifications

Immunogen

SNRPA (AAH00405, 1 a.a. ~ 282 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAVPETRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGATPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLSENPPNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAGAARDALQGFKITQNNAMKISFAKK

Specificity

SNRPA - small nuclear ribonucleoprotein polypeptide A

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for U1A Antibody (3F9-1F7) - Azide and BSA Free

Western Blot: U1A Antibody (3F9-1F7) [H00006626-M01]

Western Blot: U1A Antibody (3F9-1F7) [H00006626-M01]

Western Blot: U1A Antibody (3F9-1F7) [H00006626-M01] - SNRPA monoclonal antibody (M01), clone 3F9-1F7 Analysis of SNRPA expression in Hela.
Immunocytochemistry/ Immunofluorescence: U1A Antibody (3F9-1F7) [H00006626-M01]

Immunocytochemistry/ Immunofluorescence: U1A Antibody (3F9-1F7) [H00006626-M01]

Immunocytochemistry/Immunofluorescence: U1A Antibody (3F9-1F7) [H00006626-M01] - Analysis of monoclonal antibody to SNRPA on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: U1A Antibody (3F9-1F7) [H00006626-M01]

Immunohistochemistry-Paraffin: U1A Antibody (3F9-1F7) [H00006626-M01]

Immunohistochemistry-Paraffin: U1A Antibody (3F9-1F7) [H00006626-M01] - Analysis of monoclonal antibody to SNRPA on formalin-fixed paraffin-embedded human heart tissue. Antibody concentration 3 ug/ml.
Western Blot: U1A Antibody (3F9-1F7) [H00006626-M01]

Western Blot: U1A Antibody (3F9-1F7) [H00006626-M01]

Western Blot: U1A Antibody (3F9-1F7) [H00006626-M01] - Analysis of SNRPA expression in transfected 293T cell line by SNRPA monoclonal antibody (M01), clone 3F9-1F7.Lane 1: SNRPA transfected lysate(31.3 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: U1A Antibody (3F9-1F7) [H00006626-M01] - Detection limit for recombinant GST tagged SNRPA is approximately 0.03ng/ml as a capture antibody.

Applications for U1A Antibody (3F9-1F7) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: U1A

U1A binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and p

Alternate Names

Mud1, small nuclear ribonucleoprotein polypeptide A, U1 snRNP A, U1 snRNP-specific protein A, U1-AU1 small nuclear ribonucleoprotein A, U1AU1 small nuclear RNP-specific A

Entrez Gene IDs

6626 (Human)

Gene Symbol

SNRPA

OMIM

182285 (Human)

UniProt

AAH00405 (Human)

Additional U1A Products

Product Documents for U1A Antibody (3F9-1F7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for U1A Antibody (3F9-1F7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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