UHRF1 Antibody

Novus Biologicals | Catalog # NB100-74597

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all UHRF1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human nuclear zinc finger protein Np95 (ubiquitin-like, containing PHD and RING finger domains, 1 ) using the numbering given in entry NP_001041666.1 (GeneID 29128).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UHRF1 Antibody

Western Blot: UHRF1 Antibody [NB100-74597]

Western Blot: UHRF1 Antibody [NB100-74597]

Western Blot: UHRF1 Antibody [NB100-74597] - Detection of Human NP95/UHRF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NP95/UHRF1 antibody NB100-74597 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: UHRF1 Antibody [NB100-74597]

Immunohistochemistry: UHRF1 Antibody [NB100-74597]

Immunohistochemistry: UHRF1 Antibody [NB100-74597] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- NP95/UHRF1 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: UHRF1 Antibody [NB100-74597]

Western Blot: UHRF1 Antibody [NB100-74597]

Western Blot: UHRF1 Antibody [NB100-74597] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74597 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.
Immunohistochemistry-Paraffin: UHRF1 Antibody [NB100-74597]

Immunohistochemistry-Paraffin: UHRF1 Antibody [NB100-74597]

Immunohistochemistry-Paraffin: UHRF1 Antibody [NB100-74597] - FFPE section of human basal cell carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: UHRF1 Antibody [NB100-74597]

Immunohistochemistry-Paraffin: UHRF1 Antibody [NB100-74597]

Immunohistochemistry-Paraffin: UHRF1 Antibody [NB100-74597] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunoprecipitation: UHRF1 Antibody [NB100-74597]

Immunoprecipitation: UHRF1 Antibody [NB100-74597]

Immunoprecipitation: UHRF1 Antibody [NB100-74597] - Detection of human NP95/UHRF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NP95/UHRF1 antibody NB100-74597 (lot NB100-74597-3) used for IP at 6 ug per reaction. NP95/UHRF1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-74597-2). For blotting immunoprecipitated NP95/UHRF1, NB100-74597 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for UHRF1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:250

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: UHRF1

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase II alpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.

Long Name

E3 ubiquitin-protein ligase UHRF1

Alternate Names

hNp95, hUHRF1, HuNp95, ICBP90, NP95, Nuclear protein 95, RNF106

Entrez Gene IDs

29128 (Human)

Gene Symbol

UHRF1

UniProt

Q96T88 (Human)

Additional UHRF1 Products

Product Documents for UHRF1 Antibody

Certificate of Analysis

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Product Specific Notices for UHRF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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