UNK Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-89596
![UNK Antibody - BSA Free Western Blot: UNK Antibody - BSA Free [NBP1-89596]](https://resources.rndsystems.com/images/products/nbp1-89596_rabbit-polyclonal-unk-antibody-15520258273081.jpg "Western Blot: UNK Antibody - BSA Free [NBP1-89596]")
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Cited:
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DAWKKEAEEAGERASAAGAECELAREQRDALEVQVKKLQEELERLHAGPEPQALPAFSDLEALSLSTLYSLQKQLRAHLEQVDKAVFHMQSVKCLKCQEQKRAVL
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%). Mouse, Human reactivity reported in scientific literature (PMID: 25737280).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for UNK Antibody - BSA Free
![UNK Antibody - BSA Free Immunohistochemistry-Paraffin: UNK Antibody - BSA Free [NBP1-89596]](https://resources.rndsystems.com/images/products/nbp1-89596_rabbit-polyclonal-unk-antibody-1552025825113.jpg "Immunohistochemistry-Paraffin: UNK Antibody - BSA Free [NBP1-89596]")
Immunohistochemistry-Paraffin: UNK Antibody - BSA Free [NBP1-89596]
Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.![UNK Antibody - BSA Free Immunohistochemistry-Paraffin: UNK Antibody - BSA Free [NBP1-89596]](https://resources.rndsystems.com/images/products/nbp1-89596_rabbit-polyclonal-unk-antibody-13520251661873.jpg "Immunohistochemistry-Paraffin: UNK Antibody - BSA Free [NBP1-89596]")
Immunohistochemistry-Paraffin: UNK Antibody - BSA Free [NBP1-89596]
Staining of human liver shows no positivity in hepatocytes as expected.![UNK Antibody - BSA Free Immunohistochemistry-Paraffin: UNK Antibody - BSA Free [NBP1-89596]](https://resources.rndsystems.com/images/products/nbp1-89596_rabbit-polyclonal-unk-antibody-155202582449.jpg "Immunohistochemistry-Paraffin: UNK Antibody - BSA Free [NBP1-89596]")
Immunohistochemistry-Paraffin: UNK Antibody - BSA Free [NBP1-89596]
Staining of human colon shows weak cytoplasmic positivity in glandular cells.![UNK Antibody - BSA Free Immunohistochemistry-Paraffin: UNK Antibody - BSA Free [NBP1-89596]](https://resources.rndsystems.com/images/products/nbp1-89596_rabbit-polyclonal-unk-antibody-1552025825118.jpg "Immunohistochemistry-Paraffin: UNK Antibody - BSA Free [NBP1-89596]")
Immunohistochemistry-Paraffin: UNK Antibody - BSA Free [NBP1-89596]
Staining of human kidney shows weak cytoplasmic positivity in cells in tubules.Applications for UNK Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. IP, WB reactivity reported in (PMID: 26641712).
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UNK
Alternate Names
UNK unkempt homolog (Drosophila), ZC3H5, ZC3HDC5
Gene Symbol
UNK
UniProt
Additional UNK Products
Product Documents for UNK Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UNK Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for UNK Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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