USP45 Antibody (1H2) - Azide and BSA Free
Novus Biologicals | Catalog # H00085015-M01
![Western Blot: USP45 Antibody (1H2) [H00085015-M01]](https://resources.rndsystems.com/images/products/USP45-Antibody-1H2-Western-Blot-H00085015-M01-img0008.jpg "Western Blot: USP45 Antibody (1H2) [H00085015-M01]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1H2
Format
Azide and BSA Free
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Product Specifications
Immunogen
USP45 (XP_371838, 106 a.a. ~ 196 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FKSSRTEPHCIIINLSTWIIWCYECDEKLSTHCNKKVLAQIVDFLQKHASKTQTSAFSRIMKLCEEKCETDEIQKGGKCRNLSVRGITNLG
Specificity
USP45 - ubiquitin specific peptidase 45 (1H2)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for USP45 Antibody (1H2) - Azide and BSA Free
Western Blot: USP45 Antibody (1H2) [H00085015-M01]
Western Blot: USP45 Antibody (1H2) [H00085015-M01] - USP45 monoclonal antibody (M01), clone 1H2. Analysis of USP45 expression in NIH/3T3.![Immunohistochemistry-Paraffin: USP45 Antibody (1H2) [H00085015-M01]](https://resources.rndsystems.com/images/products/USP45-Antibody-1H2-Immunohistochemistry-Paraffin-H00085015-M01-img0003.jpg "Immunohistochemistry-Paraffin: USP45 Antibody (1H2) [H00085015-M01]")
Immunohistochemistry-Paraffin: USP45 Antibody (1H2) [H00085015-M01]
Immunohistochemistry-Paraffin: USP45 Antibody (1H2) [H00085015-M01] - Analysis of monoclonal antibody to USP45 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.![Western Blot: USP45 Antibody (1H2) [H00085015-M01]](https://resources.rndsystems.com/images/products/USP45-Antibody-1H2-Western-Blot-H00085015-M01-img0005.jpg "Western Blot: USP45 Antibody (1H2) [H00085015-M01]")
Western Blot: USP45 Antibody (1H2) [H00085015-M01]
Western Blot: USP45 Antibody (1H2) [H00085015-M01] - USP45 monoclonal antibody (M01), clone 1H2. Analysis of USP45 expression in HeLa.![Western Blot: USP45 Antibody (1H2) [H00085015-M01]](https://resources.rndsystems.com/images/products/USP45-Antibody-1H2-Western-Blot-H00085015-M01-img0006.jpg "Western Blot: USP45 Antibody (1H2) [H00085015-M01]")
Western Blot: USP45 Antibody (1H2) [H00085015-M01]
Western Blot: USP45 Antibody (1H2) [H00085015-M01] - USP45 monoclonal antibody (M01), clone 1H2. Analysis of USP45 expression in PC-12.![Western Blot: USP45 Antibody (1H2) [H00085015-M01]](https://resources.rndsystems.com/images/products/USP45-Antibody-1H2-Western-Blot-H00085015-M01-img0007.jpg "Western Blot: USP45 Antibody (1H2) [H00085015-M01]")
Western Blot: USP45 Antibody (1H2) [H00085015-M01]
Western Blot: USP45 Antibody (1H2) [H00085015-M01] - USP45 monoclonal antibody (M01), clone 1H2. Analysis of USP45 expression in A-431.![Western Blot: USP45 Antibody (1H2) [H00085015-M01]](https://resources.rndsystems.com/images/products/USP45-Antibody-1H2-Western-Blot-H00085015-M01-img0009.jpg "Western Blot: USP45 Antibody (1H2) [H00085015-M01]")
Western Blot: USP45 Antibody (1H2) [H00085015-M01]
Western Blot: USP45 Antibody (1H2) [H00085015-M01] - USP45 monoclonal antibody (M01), clone 1H2. Analysis of USP45 expression in human pancreas.
Sandwich ELISA: USP45 Antibody (1H2) [H00085015-M01] - Detection limit for recombinant GST tagged USP45 is 0.3 ng/ml as a capture antibody.
Applications for USP45 Antibody (1H2) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: USP45
Long Name
Ubiquitin carboxyl-terminal hydrolase 45
Alternate Names
Deubiquitinating enzyme 45, EC 3.1.2.15, EC 3.4.19.12, MGC14793, ubiquitin carboxyl-terminal hydrolase 45, ubiquitin specific peptidase 45, ubiquitin specific protease 45, ubiquitin thioesterase 45, Ubiquitin thiolesterase 45, Ubiquitin-specific-processing protease 45
Entrez Gene IDs
85015 (Human)
Gene Symbol
USP45
UniProt
Additional USP45 Products
Product Documents for USP45 Antibody (1H2) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for USP45 Antibody (1H2) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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