USP54 Antibody (OTI1G6) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-74813
![Western Blot: USP54 Antibody (OTI1G6)Azide and BSA Free [NBP2-74813]](https://resources.rndsystems.com/images/products/USP54-Antibody-OTI1G6-Azide-and-BSA-Free-Western-Blot-NBP2-74813-img0001.jpg "Western Blot: USP54 Antibody (OTI1G6)Azide and BSA Free [NBP2-74813]")
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1G6
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 358-700 of human USP54 (NP_689799) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for USP54 Antibody (OTI1G6) - Azide and BSA Free
Western Blot: USP54 Antibody (OTI1G6)Azide and BSA Free [NBP2-74813]
Western Blot: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP54.![Immunocytochemistry/ Immunofluorescence: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813]](https://resources.rndsystems.com/images/products/USP54-Antibody-OTI1G6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74813-img0003.jpg "Immunocytochemistry/ Immunofluorescence: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813]")
Immunocytochemistry/ Immunofluorescence: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813]
Immunocytochemistry/Immunofluorescence: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY USP54.![Flow Cytometry: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813]](https://resources.rndsystems.com/images/products/USP54-Antibody-OTI1G6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74813-img0002.jpg "Flow Cytometry: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813]")
Flow Cytometry: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813]
Flow Cytometry: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by USP54 antibody.Applications for USP54 Antibody (OTI1G6) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: USP54
Long Name
Inactive ubiquitin carboxyl-terminal hydrolase 54
Alternate Names
C10orf29
Gene Symbol
USP54
Additional USP54 Products
Product Documents for USP54 Antibody (OTI1G6) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for USP54 Antibody (OTI1G6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for USP54 Antibody (OTI1G6) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review USP54 Antibody (OTI1G6) - Azide and BSA Free and earn rewards!
Have you used USP54 Antibody (OTI1G6) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...