VAChT/SLC18A3 Antibody (N425/45) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-52349

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # N425/45

Format

Azide and BSA Free
View all VAChT/SLC18A3 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 55-125 (first vesicle lumen domain) of rat VAChT/SLC18A3 (Uniprot#: Q62666) produced recombinantly in E. Coli

Specificity

Does not cross-react with VMAT1 or VMAT2

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N425/45 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N425/45 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and and buffer exchanged into 1X PBS.

Scientific Data Images for VAChT/SLC18A3 Antibody (N425/45) - Chimeric - Azide and BSA Free

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody (N425/45) - Chimeric - Azide and BSA Free [NBP3-52349]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody (N425/45) - Chimeric - Azide and BSA Free [NBP3-52349]

Representative section of formalin fixed, paraffin-embedded rat brain stem showing facial motor neurons stained by NBP3-52348 (the BSA Free version of this antibody) using goat anti-chicken IgY secondary antibody (green). DAPI nuclear stain (blue) shows cell nuclei.
Western Blot: VAChT/SLC18A3 Antibody (N425/45) - Chimeric - Azide and BSA Free [NBP3-52349]

Western Blot: VAChT/SLC18A3 Antibody (N425/45) - Chimeric - Azide and BSA Free [NBP3-52349]

Western blotting of mock transfected (A) and VAChT/SLC18A3 transfected (B) COS-7 lysates with VAChT/SLC18A3 mouse monoclonal antibody and NBP3-52348 (the BSA Free version of this antibody). The mouse monoclonal antibody and chicken monoclonal antibody were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. The chicken monoclonal antibody recognizes exogenous VAChT/SLC18A3 with similar sensitivity as the mouse monoclonal antibody only in VAChT/SLC18A3 transfected COS-7 lysates at ~60 kDa.

Applications for VAChT/SLC18A3 Antibody (N425/45) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VAChT/SLC18A3

Vesicular Acetylcholine Transporter (VAChT), (approx. 70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate. VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer's disease and other nervous system disorders.

Long Name

Vesicular Acetylcholine Transporter/Solute Carrier Family 18 Member 3

Alternate Names

SLC18A3

Gene Symbol

SLC18A3

Additional VAChT/SLC18A3 Products

Product Documents for VAChT/SLC18A3 Antibody (N425/45) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for VAChT/SLC18A3 Antibody (N425/45) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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