VEGF-C Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB110-61022

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all VEGF-C Primary Antibodies »

Product Specifications

Immunogen

Rat VEGF-C full length protein

Specificity

This antibody recognizes rat VEGF-C and has strong cross-reactivity with mouse and human VEGF-C.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VEGF-C Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: VEGF-C Antibody [NB110-61022]

Immunocytochemistry/ Immunofluorescence: VEGF-C Antibody [NB110-61022]

Immunocytochemistry/Immunofluorescence: VEGF-C Antibody [NB110-61022] - bEnd.3 mouse brain endothelioma cell line stained with Vegf-c antibody at 1:500. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: VEGF-C Antibody [NB110-61022]

Immunohistochemistry-Paraffin: VEGF-C Antibody [NB110-61022]

VEGF-C-Antibody-Immunohistochemistry-Paraffin-NB110-61022-img0003.jpg
Immunohistochemistry: VEGF-C Antibody [NB110-61022]

Immunohistochemistry: VEGF-C Antibody [NB110-61022]

Immunohistochemistry: VEGF-C Antibody [NB110-61022] - VEGFC Antibody [NB110-61022] - 4% PFA fixed and paraffin embedded mouse Lung tissue section was subjected to IHC staining of mouse VEGF-C.
VEGF-C Antibody

Western Blot: VEGF-C Antibody [NB110-61022] -

Western Blot: VEGF-C Antibody [NB110-61022] - Kallikrein-related peptidase 3 (KLK3)/Prostate specific antigen (PSA) activates VEGF-C.(A, B) Cleavage of pro-VEGF-C by KLK3 (PSA). Pro-VEGF-C was incubated with or without KLK3, with & without the monoclonal antibody against KLK3 (5C7). Detection of VEGF-C in Western blots probed with antiserum 6 & 3/4, resulting in the detection of pro-VEGF-C (29/31 kDa) & activated, mature VEGF-C (21/23 kDa). The band marked by the asterisk likely represents the N-terminal propeptide (~15 kDa) which is detected by the antiserum 6. Note that for the image shown for antiserum 6, two different exposures of the same blot were merged (n = 3). (C, D) VEGF-C processed by KLK3 is biologically active in Ba/F3 cell assays, which translate activation of a hybrid VEGFR/EpoR receptor into cell survival (n = 2). Error bars indicate SD.10.7554/eLife.44478.004Figure 1—source data 1.Ba/F3 assay showing the activity of KLK3-cleaved VEGF-C.Ba/F3 assay showing the activity of KLK3-cleaved VEGF-C. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31099754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for VEGF-C Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000

Reviewed Applications

Read 1 review rated 5 using NB110-61022 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/mL.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF-C

Vascular endothelial growth factors (VEGFs), also known as vasculotropins, are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. The target cell specificity of VEGF is restricted to vascular endothelial cells. Vascular Endothelial Growth Factor C (VEGFC) is a member of the VEGF subfamily of PDGF-related growth factors. It is the ligand for Flt4 (VEGFR3) and KDR (VEGFR2). VEGFC binds Flt4 and induces tyrosine autophosphorylation of VEGFR3 and VEGFR2. VEGFC also stimulates the migration of bovine capillary endothelial cells in collagen gel. It is a specific growth factor for the lymphatic vascular system and mediates lymphangiogenesis. VEGFC is abundantly expressed in heart and skeletal muscle. Other tissues such as lung and kidney also express VEGFC. The human VEGFC gene is located on chromosome 4q34.

Long Name

Vascular Endothelial Growth Factor C

Alternate Names

VEGFC

Entrez Gene IDs

7424 (Human); 22341 (Mouse); 114111 (Rat)

Gene Symbol

VEGFC

UniProt

O35757 (Rat)

Additional VEGF-C Products

Product Documents for VEGF-C Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VEGF-C Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VEGF-C Antibody - Azide and BSA Free

Customer Reviews for VEGF-C Antibody - Azide and BSA Free (1)

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Customer Images

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  • VEGF-C Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: bEnd.3 mouse endothelioma cell line
    Species: Mouse
    Verified Customer | Posted 07/23/2020
    bend.3 stained with Vegf-c concentration 1:500
    VEGF-C Antibody - Azide and BSA Free NB110-61022
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VEGF-C Antibody - Azide and BSA Free

Showing  1 - 3 的 3 FAQs Showing All

  • Q: Do you have any data which show the molecular weight of the VEGFC antibody (NB110-61022) by western blotting?

    A: We do not currently have any images of NB110-61022 being used in Western blot, although it is guaranteed for this use. NBP1-45924 and NBP1-97842 show a band around 16 kDa. This is the predicted molecular weight of VEGF-C (UniProt P49767) and this is the weight the band using NB110-61022 should show up at.

  • Q: I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?

    A:

    We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

  • Q: What is the molecular weight for VEGFC Antibody (NB110-61022) on Western blot?

    A: The expected molecular weight of VEGFC is about 47KDa according to UniProt.

  • Q: Do you have any data which show the molecular weight of the VEGFC antibody (NB110-61022) by western blotting?

    A: We do not currently have any images of NB110-61022 being used in Western blot, although it is guaranteed for this use. NBP1-45924 and NBP1-97842 show a band around 16 kDa. This is the predicted molecular weight of VEGF-C (UniProt P49767) and this is the weight the band using NB110-61022 should show up at.

  • Q: I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?

    A:

    We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

  • Q: What is the molecular weight for VEGFC Antibody (NB110-61022) on Western blot?

    A: The expected molecular weight of VEGFC is about 47KDa according to UniProt.

  • Q: Do you have any data which show the molecular weight of the VEGFC antibody (NB110-61022) by western blotting?

    A: We do not currently have any images of NB110-61022 being used in Western blot, although it is guaranteed for this use. NBP1-45924 and NBP1-97842 show a band around 16 kDa. This is the predicted molecular weight of VEGF-C (UniProt P49767) and this is the weight the band using NB110-61022 should show up at.

  • Q: I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?

    A:

    We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

  • Q: What is the molecular weight for VEGFC Antibody (NB110-61022) on Western blot?

    A: The expected molecular weight of VEGFC is about 47KDa according to UniProt.

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