VPS35 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31069

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VPS35 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary.

Localization

Cytoplasm, Membrane, Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VPS35 Antibody - BSA Free

Immunohistochemistry-Paraffin: VPS35 Antibody [NBP1-31069]

Immunohistochemistry-Paraffin: VPS35 Antibody [NBP1-31069]

Immunohistochemistry-Paraffin: VPS35 Antibody [NBP1-31069] - VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term dilution: 1:250.
Western Blot: VPS35 Antibody [NBP1-31069]

Western Blot: VPS35 Antibody [NBP1-31069]

Western Blot: VPS35 Antibody [NBP1-31069] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: VPS35 Antibody [NBP1-31069]

Western Blot: VPS35 Antibody [NBP1-31069]

Western Blot: VPS35 Antibody [NBP1-31069] - A. 50 ug rat brain lysate/extract 7.5% SDS-PAGE VPS35 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
VPS35 Antibody

Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NBP1-31069] -

Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NBP1-31069] - VPS35 antibody [C3], C-term detects VPS35 protein at endosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS35 stained by VPS35 antibody [C3], C-term (NBP1-31069) diluted at 1:500.
Blue: Fluoroshield with DAPI.
VPS35 Antibody

Western Blot: VPS35 Antibody [NBP1-31069] -

Western Blot: VPS35 Antibody [NBP1-31069] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (NBP1-31069) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
VPS35 Antibody

Western Blot: VPS35 Antibody [NBP1-31069] -

Western Blot: VPS35 Antibody [NBP1-31069] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for VPS35 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VPS35

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Long Name

VPS35 retromer complex component

Alternate Names

MEM3, PARK17

Entrez Gene IDs

55737 (Human)

Gene Symbol

VPS35

UniProt

Q96QK1 (Human)

Additional VPS35 Products

Product Documents for VPS35 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for VPS35 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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