WRN Antibody - BSA Free

Novus Biologicals | Catalog # NB100-471

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WRN Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 400 and 450 of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191 (GeneID 7486).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WRN Antibody - BSA Free

Western Blot: WRN Antibody [NB100-471]

Western Blot: WRN Antibody [NB100-471]

Western Blot: WRN Antibody [NB100-471] - Human colon cancer cells with siRNA Control, siRNA pool of siWrn and 3 different sets of siRNA Wrn. GAPDH laoding control. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: WRN Antibody [NB100-471]

Immunocytochemistry/ Immunofluorescence: WRN Antibody [NB100-471]

Immunocytochemistry/Immunofluorescence: WRN Antibody [NB100-471] - Analysis of WRN in untreated, asynchronous HeLa cells using anti-WRN antibody. The primary antibody was used at a dilution of 1:100, incubated for 1 hour at room temperature in 1XPBS. Image from verified customer review.
Western Blot: WRN Antibody [NB100-471]

Western Blot: WRN Antibody [NB100-471]

Western Blot: WRN Antibody [NB100-471] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells.
Western Blot: WRN Antibody [NB100-471]

Western Blot: WRN Antibody [NB100-471]

Western Blot: WRN Antibody [NB100-471] - Detection of WRN in 293T whole cell lysate using NB 100-471.
Western Blot: WRN Antibody [NB100-471]

Western Blot: WRN Antibody [NB100-471]

Western Blot: WRN Antibody [NB100-471] - Detection of human WRN on HeLa whole cell lysate using NB100-471. WRN was immunoprecipitated with NB100-471 or rabbit anti-WRN antibody NB100-472.
Immunoprecipitation: WRN Antibody [NB100-471]

Immunoprecipitation: WRN Antibody [NB100-471]

Immunoprecipitation: WRN Antibody [NB100-471] - Detection of human WRN by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WRN antibody NB100-471 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WRN was also immunoprecipitated by rabbit anti-WRN antibody NB100-472, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Applications for WRN Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

2 - 5 ug/mg lysate

Simple Western

1:25

Western Blot

1:5000 - 1:25000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 25294835). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 20065033). Use in Immunohistochemistry reported in scientific literature (PMID: 30146558). WRN antibody validated for WB from a verified customer review.
See Simple Western Antibody Database for Simple Western validation: Tested in Cell Lysate, separated by Size, antibody dilution of 1:25, apparent MW was 200 kDa

Reviewed Applications

Read 2 reviews rated 4 using NB100-471 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WRN

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner Syndrome, an autosomal recessive disorder characterized by premature aging.

Long Name

Werner syndrome ATP-dependent helicase

Alternate Names

EC 3.1.-.-, Exonuclease WRN, RecQ protein-like 2, RecQ3, RECQL2

Entrez Gene IDs

7486 (Human)

Gene Symbol

WRN

UniProt

Q14191 (Human)

Additional WRN Products

Product Documents for WRN Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for WRN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for WRN Antibody - BSA Free

Customer Reviews for WRN Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
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Customer Images

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  • WRN Antibody
    Name: Ingrid MONTARRAS
    Application: Western Blot
    Sample Tested: Colon cancer cell line
    Species: Human
    Verified Customer | Posted 12/09/2019
    Human colon cancer cells with siRNA CTRL/siRNA pool of siWrn/ 3 differents siRNA Wrn GAPDH control for loading
    Ab used at 1/1000_ON
    WRN Antibody - BSA Free NB100-471
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Asynchronous HeLa cells
    Species: Human
    Verified Customer | Posted 01/12/2015
    Localization of WRN in untreated, asynchronous HeLa cells (red = WRN; blue = DAPI).
    WRN Antibody - BSA Free NB100-471

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Protocols

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