WSTF Antibody (5E9) - Azide and BSA Free
Novus Biologicals | Catalog # H00009031-M01
![Immunohistochemistry-Paraffin: WSTF Antibody (5E9) [H00009031-M01]](https://resources.rndsystems.com/images/products/WSTF-Antibody-5E9-Immunohistochemistry-Paraffin-H00009031-M01-img0005.jpg "Immunohistochemistry-Paraffin: WSTF Antibody (5E9) [H00009031-M01]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Sandwich ELISA, Proximity Ligation Assay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5E9
Format
Azide and BSA Free
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Product Specifications
Immunogen
BAZ1B (NP_075381, 1384 a.a. ~ 1483 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDFQTVQNKCSCGSYRSVQEFLTDMKQVFTNAEVYNCRGSHVLSCMVKTEQCLVALLHKHLPGHPYVRRKRKKFPDRLAEDEGDSEPEAVGQSRGRRQKK
Localization
Nuclear
Specificity
BAZ1B - bromodomain adjacent to zinc finger domain, 1B
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for WSTF Antibody (5E9) - Azide and BSA Free
Immunohistochemistry-Paraffin: WSTF Antibody (5E9) [H00009031-M01]
Immunohistochemistry-Paraffin: WSTF Antibody (5E9) [H00009031-M01] - Analysis of monoclonal antibody to BAZ1B on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.![Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]](https://resources.rndsystems.com/images/products/WSTF-Antibody-5E9-Proximity-Ligation-Assay-H00009031-M01-img0002.jpg "Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]")
Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]
Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01] - Analysis of protein-protein interactions between SMARCB1 and BAZ1B. HeLa cells were stained with anti-SMARCB1 rabbit purified polyclonal 1:1200 and anti-BAZ1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).![Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]](https://resources.rndsystems.com/images/products/WSTF-Antibody-5E9-Proximity-Ligation-Assay-H00009031-M01-img0003.jpg "Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]")
Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]
Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01] - Analysis of protein-protein interactions between SMARCB1 and BAZ1B. Huh7 cells were stained with anti-SMARCB1 rabbit purified polyclonal 1:1200 and anti-BAZ1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: WSTF Antibody (5E9) [H00009031-M01] - Detection limit for recombinant GST tagged BAZ1B is 0.3 ng/ml as a capture antibody.
Applications for WSTF Antibody (5E9) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Proximity Ligation Assay
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. It has been used for IHC-P.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: WSTF
Alternate Names
bromodomain adjacent to zinc finger domain, 1B, williams-Beuren syndrome chromosomal region 10 protein
Entrez Gene IDs
9031 (Human)
Gene Symbol
BAZ1B
OMIM
605681 (Human)
Additional WSTF Products
Product Documents for WSTF Antibody (5E9) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for WSTF Antibody (5E9) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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