XPF Antibody

Novus Biologicals | Catalog # NB100-60679

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Cited:

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all XPF Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 855 and 905 of human xeroderma pigmentosum, complementation group F using the numbering given in entry AAB07689.1 (GeneID 2072).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for XPF Antibody

Western Blot: XPF Antibody [NB100-60679]

Western Blot: XPF Antibody [NB100-60679]

Western Blot: XPF Antibody [NB100-60679] - Detection of Human XPF/ERCC4 on HeLa whole cell lysate using NB100-60679.
Immunohistochemistry-Paraffin: XPF Antibody [NB100-60679]

Immunohistochemistry-Paraffin: XPF Antibody [NB100-60679]

Immunohistochemistry-Paraffin: XPF Antibody [NB100-60679] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti- XPF/ERCC4 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for XPF Antibody

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
ELISA reactivity reported in scientific literature (PMID:20812782). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: XPF

XPF/ERCC4 is suggested to play a role in the repair of DNA double-strand breaks (DSB), homologous recombination, and gene conversion via single-strand annealing (SSA). XPF/ERCC4 is an endonuclease that incises 5-prime DNA. Defects in XPF/ERCC4 cause xeroderma pigmentosum VI (XP6) an autosomal recessive disease characterized by hypersensitivity to sunlight and a predisposition to skin cancer as well as neurological abnormalities. Defects in XPF/ERCC4 are also responsible for XFE progeroid syndrome, a syndrome characterized by dwarfism, cachexia, and microcephaly.

Long Name

Xeroderma Pigmentosum F

Alternate Names

ERCC4

Entrez Gene IDs

2072 (Human)

Gene Symbol

ERCC4

UniProt

Q92889 (Human)

Additional XPF Products

Product Documents for XPF Antibody

Certificate of Analysis

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Product Specific Notices for XPF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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